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Lookup NU author(s): George Mangse, Professor David WernerORCiD, Dr Paola Meynet
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Volatile Petroleum Hydrocarbon (VPH) class effects on soil microbial composition were investigated using two next-generation sequencing (NGS) techniques – 454 pyrosequencing and ion torrent sequencing. Microbial activity was stimulated by adding different VPH compound classes to the sandy soil in comparison with live controls without VPH addition. Microbial community structure was significantly affected by the various VPH classes. At the genus level, Rhodococcus, Desulfosporosinus, Polaromonas, Mesorhizobium and Methylibium had the highest relative abundances in the straight-chain alkane (str-alk) treated soil as compared to the control (p < 0.05, 2 sample t-tests) while Pseudomonas was more dominant in the cyclic alkane (cyc-alk) contaminated soil. Pseudonocardia was significantly higher in relative abundance in the aromatic hydrocarbon (aro-H) treated batches as compared to the control (p < 0.05, 2 sample t-tests). A non-metric multidimensional scaling (NMDS) of the Bray Curtis similarity between microbial communities in the batches revealed at least 60% similarity for each treatment and also showed that VPH class was a statistically significant factor in shaping the bacterial communities in the soil treatments (Global R = 0.861, p < 0.01). The NGS platforms (454 GS Junior and Ion torrent) compared in this study did not appear to affect the outcomes of the microbial community structure and composition analysis.
Author(s): Mangse G, Werner D, Meynet P, Ogbaga C
Publication type: Article
Publication status: Published
Journal: Environmental Pollution
Year: 2020
Volume: 264
Print publication date: 03/09/2020
Online publication date: 06/05/2020
Acceptance date: 03/05/2020
Date deposited: 08/05/2020
ISSN (print): 0269-7491
ISSN (electronic): 1873-6424
Publisher: Elsevier Ltd
URL: https://doi.org/10.1016/j.envpol.2020.114738
DOI: 10.1016/j.envpol.2020.114738
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