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Microbial community responses to different volatile petroleum hydrocarbon class mixtures in an aerobic sandy soil

Lookup NU author(s): George Mangse, Professor David Werner, Dr Paola Meynet

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).


Abstract

Volatile Petroleum Hydrocarbon (VPH) class effects on soil microbial composition were investigated using two next-generation sequencing (NGS) techniques – 454 pyrosequencing and ion torrent sequencing. Microbial activity was stimulated by adding different VPH compound classes to the sandy soil in comparison with live controls without VPH addition. Microbial community structure was significantly affected by the various VPH classes. At the genus level, Rhodococcus, Desulfosporosinus, Polaromonas, Mesorhizobium and Methylibium had the highest relative abundances in the straight-chain alkane (str-alk) treated soil as compared to the control (p < 0.05, 2 sample t-tests) while Pseudomonas was more dominant in the cyclic alkane (cyc-alk) contaminated soil. Pseudonocardia was significantly higher in relative abundance in the aromatic hydrocarbon (aro-H) treated batches as compared to the control (p < 0.05, 2 sample t-tests). A non-metric multidimensional scaling (NMDS) of the Bray Curtis similarity between microbial communities in the batches revealed at least 60% similarity for each treatment and also showed that VPH class was a statistically significant factor in shaping the bacterial communities in the soil treatments (Global R = 0.861, p < 0.01). The NGS platforms (454 GS Junior and Ion torrent) compared in this study did not appear to affect the outcomes of the microbial community structure and composition analysis.


Publication metadata

Author(s): Mangse G, Werner D, Meynet P, Ogbaga C

Publication type: Article

Publication status: Published

Journal: Environmental Pollution

Year: 2020

Volume: 264

Print publication date: 03/09/2020

Online publication date: 06/05/2020

Acceptance date: 03/05/2020

Date deposited: 08/05/2020

ISSN (print): 0269-7491

ISSN (electronic): 1873-6424

Publisher: Elsevier Ltd

URL: https://doi.org/10.1016/j.envpol.2020.114738

DOI: 10.1016/j.envpol.2020.114738


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