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Aminoacyl-TRNa synthetases and tRNAS for an expanded genetic code: What makes them orthogonal?

Lookup NU author(s): Dr Sergey MelnikovORCiD


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© 2019 by the authors. Licensee MDPI, Basel, Switzerland.In the past two decades, tRNA molecules and their corresponding aminoacyl-tRNA synthetases (aaRS) have been extensively used in synthetic biology to genetically encode post-translationally modified and unnatural amino acids. In this review, we briefly examine one fundamental requirement for the successful application of tRNA/aaRS pairs for expanding the genetic code. This requirement is known as “orthogonality”—the ability of a tRNA and its corresponding aaRS to interact exclusively with each other and avoid cross-reactions with additional types of tRNAs and aaRSs in a given organism.

Publication metadata

Author(s): Melnikov SV, Soll D

Publication type: Review

Publication status: Published

Journal: International Journal of Molecular Sciences

Year: 2019

Volume: 20

Issue: 8

Pages: 1929

Online publication date: 19/04/2020

Acceptance date: 17/04/2020

ISSN (print): 1661-6596

ISSN (electronic): 1422-0067

Publisher: MDPI AG


DOI: 10.3390/ijms20081929

PubMed id: 31010123