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Peeling back the layers of crassulacean acid metabolism: functional differentiation between Kalanchoë fedtschenkoi epidermis and mesophyll proteomes

Lookup NU author(s): Natalia Hurtado Castaño, Dr Danny Cowan-Turner, Emeritus Professor Jerry Barnes, Emerita Professor Anne Borland



© 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons LtdCrassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that offers the potential to engineer improved water-use efficiency (WUE) and drought resilience in C3 plants while sustaining productivity in the hotter and drier climates that are predicted for much of the world. CAM species show an inverted pattern of stomatal opening and closing across the diel cycle, which conserves water and provides a means of maintaining growth in hot, water-limited environments. Recent genome sequencing of the constitutive model CAM species Kalanchoë fedtschenkoi provides a platform for elucidating the ensemble of proteins that link photosynthetic metabolism with stomatal movement, and that protect CAM plants from harsh environmental conditions. We describe a large-scale proteomics analysis to characterize and compare proteins, as well as diel changes in their abundance in guard cell-enriched epidermis and mesophyll cells from leaves of K. fedtschenkoi. Proteins implicated in processes that encompass respiration, the transport of water and CO2, stomatal regulation, and CAM biochemistry are highlighted and discussed. Diel rescheduling of guard cell starch turnover in K. fedtschenkoi compared with that observed in Arabidopsis is reported and tissue-specific localization in the epidermis and mesophyll of isozymes implicated in starch and malate turnover are discussed in line with the contrasting roles for these metabolites within the CAM mesophyll and stomatal complex. These data reveal the proteins and the biological processes enriched in each layer and provide key information for studies aiming to adapt plants to hot and dry environments by modifying leaf physiology for improved plant sustainability.

Publication metadata

Author(s): Abraham PE, Hurtado Castano N, Cowan-Turner D, Barnes J, Poudel S, Hettich R, Flutsch S, Santelia D, Borland AM

Publication type: Article

Publication status: Published

Journal: Plant Journal

Year: 2020

Volume: 103

Issue: 2

Pages: 869-888

Online publication date: 21/04/2020

Acceptance date: 23/03/2020

Date deposited: 17/12/2020

ISSN (print): 0960-7412

ISSN (electronic): 1365-313X

Publisher: Blackwell Publishing Ltd


DOI: 10.1111/tpj.14757


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Funder referenceFunder name
31003A‐166539, 1
Biotechnology and Biological Sciences Research Council
Departamento Administrativo de Ciencia, Tecnología e Innovación
Oak Ridge National Laboratory
Office of Science