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Compact, modular and in-plane AOSLO for high-resolution retinal imaging

Lookup NU author(s): Dr Laura YoungORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2018 Optical Society of America.The adaptive optics scanning laser ophthalmoscope (AOSLO) was first developed in 2002 and since then the technology has been adopted in several laboratories around the world, for both clinical and psychophysical research. There have been a few major design implementations of the AOSLO. The first used on-axis tilted spherical mirrors in a planar arrangement, and the second minimized the build up of astigmatism present in the first design by using a non-planar arrangement. Other designs have avoided astigmatism by using custom-made toroidal mirrors or by using lenses on-axis, rather than mirrors. We present a new design implementation for an AOSLO that maintains a planar optical alignment without the build up astigmatism using compact, reconfigurable modules based on an Offner relay system. We additionally use an off-the-shelf digital oscilloscope for data capture and custom-written Python code for generating and analyzing the retinal images. This design results in a compact system that is simple to align and, being composed of modular relays, has the potential for additional components to be added. We show that this system maintains diffraction-limited image quality across the field of view and that cones are clearly resolved in the central retina. The modular relay design is generally applicable to any system requiring one or more components in the pupil conjugate plane. This is likely to be useful for any point-scanned system, such as a standard scanning laser ophthalmoscope or non-ophthalmic confocal imaging system.

Publication metadata

Author(s): Young LK, Morris TJ, Saunter CD, Smithson HE

Publication type: Article

Publication status: Published

Journal: Biomedical Optics Express

Year: 2018

Volume: 9

Issue: 9

Pages: 4275-4293

Print publication date: 01/09/2018

Online publication date: 15/08/2018

Acceptance date: 15/05/2018

Date deposited: 28/05/2020

ISSN (electronic): 2156-7085

Publisher: OSA - The Optical Society


DOI: 10.1364/BOE.9.004275


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Funder referenceFunder name
EPA Cephalosporin Fund (CF 277);
Fight For Sight (1467/8);
John Fell Oxford University Press (OUP) ResearchFund (103/786 and 151/139).
University of Oxford Medical Research Fund (MRF/LSV2015/2161)
University of Oxford Wellcome Trust Institutional Strategic SupportFund (105605/Z/14/Z);