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Non-invasive in vivo quantification of the developing optical properties and graded index of the embryonic eye lens using SPIM

Lookup NU author(s): Dr Laura YoungORCiD, Dr Miguel Jarrin



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.Graded refractive index lenses are inherent to advanced visual systems in animals. By understanding their formation and local optical properties, significant potential for improved ocular healthcare may be realized. We report a novel technique measuring the developing optical power of the eye lens, in a living animal, by exploiting the orthogonal imaging modality of a selective plane illumination microscope (SPIM). We have quantified the maturation of the lenticular refractive index at three different visible wavelengths using a combined imaging and ray tracing approach. We demonstrate that the method can be used with transgenic and vital dye labeling as well as with both fixed and living animals. Using a key eye lens morphogen and its inhibitor, we have measured their effects both on lens size and on refractive index. Our technique provides insights into the mechanisms involved in the development of this natural graded index micro-lens and its associated optical properties.

Publication metadata

Author(s): Young LK, Jarrin M, Saunter CD, Quinlan RA, Girkin JM

Publication type: Article

Publication status: Published

Journal: Biomedical Optics Express

Year: 2018

Volume: 9

Issue: 5

Pages: 2176-2188

Print publication date: 01/05/2018

Online publication date: 10/04/2018

Acceptance date: 23/03/2018

Date deposited: 28/05/2020

ISSN (electronic): 2156-7085

Publisher: Optical Society of America


DOI: 10.1364/BOE.9.002176


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Funder referenceFunder name
EPSRC (EP/M010767/1, EP I010173/1).
Fight for Sight UK (1584/1585);
Leverhulme Trust (RPG-2012-554);