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Using SPIM to track the development of the focal power of the zebrafish lens

Lookup NU author(s): Dr Laura YoungORCiD, Dr Miguel Jarrin

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Abstract

© 2015 SPIE.Selective plane illumination microscopy (SPIM) is a 3D imaging technique that uses a sheet of light to optically section a sample in vivo. A cylindrical lens focuses collimated light in one dimension, producing a sheet that is formed in the sample via an objective lens. Any optical power within the sample will additionally refract the light sheet passing through it. We exploit this effect to track the development of the optical power of the zebrafish lens over the first 4 days post fertilisation (dpf). We show that light is focussed on to the photoreceptor layer of the retina at 4 dpf.

Publication metadata

Author(s): Young LK, Jarrin M, Saunter CD, Quinlan R, Girkin JM

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Optical Methods in Developmental Biology III

Year of Conference: 2015

Pages: 933408

Online publication date: 10/03/2015

Acceptance date: 01/01/1900

ISSN: 1605-7422

Publisher: SPIE

URL: https://doi.org/10.1117/12.2079887

DOI: 10.1117/12.2079887

Library holdings: Search Newcastle University Library for this item

Series Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE

ISBN: 9781628414240

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