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Cell ablation in a single plane illumination microscope

Lookup NU author(s): Dr Laura YoungORCiD


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© OSA 2016.We have developed a Single Plane Illumination Microscope with ability to ablate single or groups of cells either through a photo-activated dye or by direct cell ablation. By integrating the ablation with heart synchronization we have ablated cells in a beating heart and observed the subsequent recovery within a zebrafish. Using a transgenic fish line containing the photo-activated "killer-red" compound we have used a Bessel beam, launched through the imaging arm of a SPIM system, to target killer-red expressing cells in the kidney. By integrating advanced optical techniques with transgenic methods the instrument developed has opened up a new route to study organ development, and repair.

Publication metadata

Author(s): Girkin JM, Buckley CB, Torres-Carvalho M, Young L, Rider S, McFadden C, Berlage C, Verdon R, Taylor J, Mullins JJ

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Frontiers in Optics 2016

Year of Conference: 2016

Acceptance date: 02/04/2016

Publisher: OSA - The Optical Society


DOI: 10.1364/FIO.2016.FF3A.4

Notes: paper FF3A.4.

Library holdings: Search Newcastle University Library for this item

Series Title: Optics InfoBase Conference Papers

ISBN: 9781943580194