Cell ablation in a single plane illumination microscope

Girkin, JM; Buckley, CB; Torres-Carvalho, M; Young, L; Rider, S; McFadden, C; Berlage, C; Verdon, R; Taylor, J; Mullins, JJ

doi:10.1364/FIO.2016.FF3A.4

Cell ablation in a single plane illumination microscope

Lookup NU author(s): Dr Laura Young ORCiD

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Abstract

© OSA 2016.We have developed a Single Plane Illumination Microscope with ability to ablate single or groups of cells either through a photo-activated dye or by direct cell ablation. By integrating the ablation with heart synchronization we have ablated cells in a beating heart and observed the subsequent recovery within a zebrafish. Using a transgenic fish line containing the photo-activated "killer-red" compound we have used a Bessel beam, launched through the imaging arm of a SPIM system, to target killer-red expressing cells in the kidney. By integrating advanced optical techniques with transgenic methods the instrument developed has opened up a new route to study organ development, and repair.