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Cell-free protein synthesis in hydrogel materials

Lookup NU author(s): Dr Colette Whitfield, Dr Alice BanksORCiD, Dr Gema Dura, Dr David Fulton, Dr Thomas HowardORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthesis.

Publication metadata

Author(s): Whitfield CJ, Banks AM, Dura G, Love J, Fieldsend JE, Goodchild SA, Fulton DA, Howard TP

Publication type: Article

Publication status: Published

Journal: Chemical Communications

Year: 2020

Volume: 56

Issue: 52

Pages: 7108-7111

Print publication date: 04/07/2020

Online publication date: 27/05/2020

Acceptance date: 19/05/2020

Date deposited: 27/05/2020

ISSN (print): 1359-7345

ISSN (electronic): 1364-548X

Publisher: Royal Society of Chemistry


DOI: 10.1039/D0CC02582H


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Funder referenceFunder name
BB/M018318/1Biotechnology and Biological Sciences Research Council (BBSRC)