Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment

Mallett, G; Patterson, W; Payne, M; Amarnath, S

doi:10.1007/978-1-0716-0338-3_14

Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment

Lookup NU author(s): Grace Mallett, Warren Patterson, Megan Payne, Dr Shoba Amarnath ORCiD

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Abstract

© 2020, Springer Science+Business Media, LLC, part of Springer Nature.Innate lymphoid cells (ILCs) are important for both tissue immunity and tissue homeostasis. They are classified into three groups: Group 1 ILCs include NK cells, which are important in eliciting immunity against intracellular pathogens; group 2 ILCs protect against parasitic helminths; and group 3 ILCs protect against extracellular pathogens. The role of ILCs in cancer immunity remains unclear. In this chapter, we discuss methods for isolating and characterizing tumor-infiltrating ILC subsets within the tumor microenvironment in an experimental murine model of B16 melanoma. The chapter also highlights the expression of PD-1 on the various ILC subsets within the tumor microenvironment.