Toggle Main Menu Toggle Search

Open Access padlockePrints

Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries

Lookup NU author(s): Dr Steve Boneham

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Affinity selection of phage display peptide libraries is routinely used for isolating peptides capable of binding a range of molecules, including antibodies and receptors. This process is most successful when the selecting molecule is relatively pure, for example, a monoclonal antibody. However, isolation of peptides able to bind to target molecules present in a complex mixture is more difficult because the affinity selection process isolates peptides capable of binding to all molecules present in the mixture. Here we describe the development of a tagged polymerase chain reaction (PCR) subtractive hybridization method that is universally applicable for the targeted isolation of peptides able to bind to unique molecules within a complex mixture. We also describe a discriminatory limiting dilution PCR method that can be used to optimize hybridization conditions. © 2004 Elsevier Inc. All rights reserved.


Publication metadata

Author(s): Tarr AW, Boneham SP, Grabowska AM, Ball JK

Publication type: Article

Publication status: Published

Journal: Analytical Biochemistry

Year: 2005

Volume: 339

Issue: 1

Pages: 61-68

Print publication date: 01/04/2005

Online publication date: 12/01/2005

ISSN (print): 0003-2697

ISSN (electronic): 1096-0309

Publisher: Elsevier

URL: https://doi.org/10.1016/j.ab.2004.12.020

DOI: 10.1016/j.ab.2004.12.020

PubMed id: 15766711


Altmetrics

Altmetrics provided by Altmetric


Share