Lookup NU author(s): Dr Steve Boneham
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Affinity selection of phage display peptide libraries is routinely used for isolating peptides capable of binding a range of molecules, including antibodies and receptors. This process is most successful when the selecting molecule is relatively pure, for example, a monoclonal antibody. However, isolation of peptides able to bind to target molecules present in a complex mixture is more difficult because the affinity selection process isolates peptides capable of binding to all molecules present in the mixture. Here we describe the development of a tagged polymerase chain reaction (PCR) subtractive hybridization method that is universally applicable for the targeted isolation of peptides able to bind to unique molecules within a complex mixture. We also describe a discriminatory limiting dilution PCR method that can be used to optimize hybridization conditions. © 2004 Elsevier Inc. All rights reserved.
Author(s): Tarr AW, Boneham SP, Grabowska AM, Ball JK
Publication type: Article
Publication status: Published
Journal: Analytical Biochemistry
Print publication date: 01/04/2005
Online publication date: 12/01/2005
ISSN (print): 0003-2697
ISSN (electronic): 1096-0309
PubMed id: 15766711
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