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Lookup NU author(s): Dr Birgit Koch, Jonathan Tellechea Luzardo, Professor Natalio KrasnogorORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2020 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. Bacterial type IV secretion systems (T4SSs) can mediate conjugation. The T4SS from Neisseria gonorrhoeae possesses the unique ability to mediate DNA secretion into the extracellular environment. The N. gonorrhoeae T4SS can be grouped with F-type conjugative T4SSs based on homology. We tested 17 proteins important for DNA secretion by N. gonorrhoeae for protein interactions. The BACTH-TM bacterial two-hybrid system was successfully used to study periplasmic interactions. By determining if the same interactions were observed for F-plasmid T4SS proteins and when one interaction partner was replaced by the corresponding protein from the other T4SS, we aimed to identify features associated with the unique function of the N. gonorrhoeae T4SS as well as generic features of F-type T4SSs. For both systems, we observed already described interactions shared by homologs from other T4SSs as well as new and described interactions between F-type T4SS-specific proteins. Furthermore, we demonstrate, for the first-time, interactions between proteins with homology to the conserved T4SS outer membrane core proteins and F-type-specific proteins and we confirmed two of them by co-purification. The F-type-specific protein TraHN was found to localize to the outer membrane and the presence of significant amounts of TraHN in the outer membrane requires TraGN.
Author(s): Koch B, Callaghan MM, Tellechea-Luzardo J, Seeger AY, Dillard JP, Krasnogor N
Publication type: Article
Publication status: Published
Journal: Molecular Microbiology
Year: 2020
Volume: 114
Issue: 5
Pages: 823-838
Print publication date: 01/11/2020
Online publication date: 01/08/2020
Acceptance date: 20/07/2020
Date deposited: 15/10/2020
ISSN (print): 0950-382X
ISSN (electronic): 1365-2958
Publisher: John Wiley & Sons Ltd
URL: https://doi.org/10.1111/mmi.14582
DOI: 10.1111/mmi.14582
PubMed id: 32738086
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