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Lookup NU author(s): Dr Naomi Willis, Professor John Mathers
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Scope: Metabolites derived from specific foods present in urine samples can provide objective biomarkers of food intake (BFIs). This study investigated the possibility that calystegines (a class of iminosugars) may provide BIFs for potato (Solanum tuberosum L.) product exposure. Methods and results: Calystegine content is examined in published data covering a wide range of potato cultivars. Rapid methods are developed for the quantification of calystegines in cooked potato products and human urine using triple quadrupole mass spectrometry. The potential of calystegines as BFIs for potato consumption is assessed in a controlled food intervention study in the United Kingdom and validated in an epidemiological study in Portugal. Calystegine concentrations are reproducibly above the quantification limit in first morning void urines the day after potato consumption, showing a good doseāresponse relationship, particularly for calystegine A3. The design of the controlled intervention mimicks exposure to a typical UK diet and showed that neither differences in preparation/cooking method or influence of other foods in the diet has significant impact on biomarker performance. Calystegine biomarkers also perform well in the independent validation study. Conclusion: It is concluded that calystegines have many of the characteristics needed to be considered as specific BFIs for potato product intake.
Author(s): Beckmann M, Lloyd AJ, Wilson T, Torres D, Goios A, Willis ND, Lyons L, Phillips H, Mathers JC, Nash RJ, Sharp H, Draper J
Publication type: Article
Publication status: Published
Journal: Molecular Nutrition & Food Research
Year: 2020
Volume: 64
Issue: 20
Print publication date: 01/10/2020
Online publication date: 11/09/2020
Acceptance date: 11/09/2020
Date deposited: 14/10/2020
ISSN (print): 1613-4125
ISSN (electronic): 1613-4133
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
URL: https://doi.org/10.1002/mnfr.202000515
DOI: 10.1002/mnfr.202000515
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