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Lookup NU author(s): Professor Mark GeogheganORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Direct glycan–glycan interactions are increasingly implicated in survival and pathogenicity of bacteria. Here, we show that they can be exploited by protozoan parasites in their insect hosts. Force spectroscopy revealed that Leishmania promastigotes display a high-affinity biomolecular interaction between their lipophosphoglycan glycocalyx and mimics of N-acetyl-D-galactosamine, commonly expressed on the midguts of a wide range of sand fly vector species. This enabled gut-adhesive nectomonad promastigotes of Leishmania mexicana to efficiently bind to membrane-bound mucin-like, O-linked glycoproteins of the sand fly Lutzomyia longipalpis, an event crucial for parasite survival, and accounts for a permissive mode of binding. Thus, direct interaction between parasite and sand fly midgut glycans are key to permitting vector competence for all forms of leishmaniasis worldwide. In addition, these studies demonstrate the feasibility of interfering with these interactions as transmission-blocking vaccines.
Author(s): Hall AR, Blakeman JT, Eissa AM, Chapman P, Morales-García AL, Stennett L, Martin O, Giraud E, Dockrell DH, Cameron NR, Wiese M, Yakob Y, Rogers ME, Geoghegan M
Publication type: Article
Publication status: Published
Journal: Chemical Science
Print publication date: 28/10/2020
Online publication date: 03/09/2020
Acceptance date: 02/09/2020
Date deposited: 21/10/2020
ISSN (print): 2041-6520
ISSN (electronic): 2041-6539
Publisher: Royal Society of Chemistry
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