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Lookup NU author(s): Dr Fiona MalcomsonORCiD,
Dr Naomi Willis,
Dr Long Xie,
Dr Betul Kocaadam,
Dr Aycil Ozturan,
Professor John Mathers
This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Association for Cancer Research, 2021.
For re-use rights please refer to the publisher's terms and conditions.
Inflammation drives colorectal cancer (CRC) development and CRC risk is influenced by dietary factors, including dietary fibre. Hyperactive WNT signalling occurs in CRC and may regulate inflammation. This study investigated i) relationships between the inflammatory potential of diet, assessed using the Energy-adjusted Dietary Inflammatory Index (E-DIITM), and markers of WNT signalling, and ii) whether DII status modulated the response to supplementation with two types of dietary fibre. Seventy-five healthy participants were supplemented with resistant starch (RS) and/or polydextrose (PD) or placebo for 50 days. Rectal biopsies were collected pre and post-intervention and used to assess WNT pathway gene expression and crypt cell proliferation. E-DII scores were calculated from food frequency questionnaire data. High-sensitivity C-reactive protein (hsCRP) and faecal calprotectin concentrations were quantified. hsCRP concentration was significantly greater in participants with higher E-DII scores (least square means (LSM) 4.7 vs. 2.4mg/L, P=0.03). Baseline E-DII score correlated with FOSL1 ( 0.503, P=0.003) and WNT11 (0.472, P=0.006) expression, after adjusting for age, gender, BMI, endoscopy procedure and smoking status. WNT11 expression was more than two-fold greater in individuals with higher E-DII scores (LSM 0.131 vs. 0.059, P=0.002). Baseline E-DII modulated the effects of PD supplementation on FOSL1 expression (P=0.04).More pro-inflammatory diets were associated with altered WNT signalling and appeared to modulate the effects of PD supplementation on expression of FOSL1.This is the first study to investigate relationships between the E-DII and molecular markers of WNT signalling in rectal tissue of healthy individuals.
Author(s): Malcomson FC, Willis ND, McCallum I, Xie L, Shivappa N, Wirth MD, Hébert JR, Kocaadam-Bozkurt B, Özturan-Sirin A, Kelly SB, Bradburn DM, Belshaw NJ, Johnson IT, Mathers JC
Publication type: Article
Publication status: Published
Journal: Cancer Prevention Research
Print publication date: 01/03/2021
Online publication date: 28/10/2020
Acceptance date: 22/10/2020
Date deposited: 11/11/2020
ISSN (print): 1940-6207
ISSN (electronic): 1940-6215
Publisher: American Association for Cancer Research
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