Toggle Main Menu Toggle Search

Open Access padlockePrints

Hepatoprotective Effects of Selenium-Enriched Probiotics Supplementation on Heat-Stressed Wistar Rat Through Anti-Inflammatory and Antioxidant Effects

Lookup NU author(s): Dr Kevin WaldronORCiD



This is the authors' accepted manuscript of an article that has been published in its final definitive form by Humana Press Inc., 2021.

For re-use rights please refer to the publisher's terms and conditions.


© 2020, Springer Science+Business Media, LLC, part of Springer Nature. The purpose of this study was to elucidate the effects of selenium-enriched probiotics on the liver of heat-stressed Wistar rats. Ten-week-old male rats were assigned to four groups: control (Con); high temperature (HT); high temperature plus probiotics (HT + P: 1011 CFU/mL Lactobacillus acidophilus and 109 CFU/mL Saccharomyces cerevisiae); or high temperature plus selenium-enriched probiotics (HT + SeP: 0.3 mg/kg Se, 1011 CFU/mL L. acidophilus and 109 CFU/mL S. cerevisiae). The HT, HT + P, and HT + SeP groups were maintained at higher ambient temperature (40–42 °C), while the control group was kept at room temperature (25 °C). After 42 days of thermal exposure, blood and liver tissues were collected and analyzed for morphological and molecular markers of liver physiology. The body weight of rats in the HT group decreased but liver weight and live index were increased. Histological examination showed dilation of liver sinusoids and congestion of interstitial veins in HT group. Moreover, the histomorphology of the liver in HT + P and HT + SeP groups was restored, and the serum AST, ALT, ALP, LDH, and hepatic MDA level decreased significantly, but the serum total protein level and the liver SOD, T-AOC, and GSH-PX activities were increased significantly relative to the HT group. In addition, the mRNA level of Gpx1, SOD1, Nrf2, and Bcl-2 was significantly increased, while the expression level of Bax, IL-6, TNF-α, COX-2, NF-κB, α-SMA, TGFβ1, Collagen I, HSP70, and HSP90 was significantly decreased in liver tissues after SeP supplementation. We concluded that SeP can protect Wistar rats from oxidative stress, inflammation, apoptosis, and liver fibrosis induced by heat stress.

Publication metadata

Author(s): Malyar RM, Naseri E, Li H, Ali I, Farid RA, Liu D, Maroof K, Nasim M, Banuree SAH, Huang K, Waldron KJ, Chen X

Publication type: Article

Publication status: Published

Journal: Biological Trace Element Research

Year: 2021

Volume: 199

Pages: 3445-3456

Print publication date: 01/09/2021

Online publication date: 07/11/2020

Acceptance date: 03/11/2020

Date deposited: 21/12/2020

ISSN (print): 0163-4984

ISSN (electronic): 1559-0720

Publisher: Humana Press Inc.


DOI: 10.1007/s12011-020-02475-3


Altmetrics provided by Altmetric