Toggle Main Menu Toggle Search

Open Access padlockePrints

D-Phenylglycine aminotransferase (d-PhgAT)-substrate scope and structural insights of a stereo-inverting biocatalyst used in the preparation of aromatic amino acids

Lookup NU author(s): Dr Jon Marles-WrightORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© The Royal Society of Chemistry.Enantiopure amines are key building blocks in the synthesis of many pharmaceuticals, so a route to their production is a current goal for biocatalysis. The stereo-inverting d-phenylglycine aminotransferase (d-PhgAT), isolated from Pseudomonas stutzeri ST-201, catalyses the reversible transamination from l-glutamic acid to benzoylformate, yielding α-ketoglutarate and d-phenylglycine (d-Phg). Detailed kinetic analysis revealed a range of amine donor and acceptor substrates that allowed the synthesis of enantiopure aromatic d-amino acids at a preparative scale. We also determined the first X-ray crystal structure of d-PhgAT with its bound pyridoxal 5′-phosphate (PLP) cofactor at 2.25 Å resolution. A combination of structural analysis and site-directed mutagenesis of this class III aminotransferase revealed key residues that are potentially involved in the dual substrate recognition, as well as controlling the stereo-inverting behaviour of d-PhgAT. Two arginine residues (Arg34 and Arg407) are involved in substrate recognition within P and O binding pockets respectively. These studies lay the foundation for further enzyme engineering and promote d-PhgAT as a useful biocatalyst for the sustainable production of high value, aromatic d-amino acids. This journal is

Publication metadata

Author(s): Serpico A, De Cesare S, Marles-Wright J, Akhtar MK, Loake GJ, Campopiano DJ

Publication type: Article

Publication status: Published

Journal: Catalysis Science and Technology

Year: 2020

Volume: 10

Issue: 19

Pages: 6533-6543

Online publication date: 28/07/2020

Acceptance date: 27/07/2020

Date deposited: 11/12/2020

ISSN (print): 2044-4753

ISSN (electronic): 2044-4761

Publisher: Royal Society of Chemistry


DOI: 10.1039/d0cy01391a


Altmetrics provided by Altmetric