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Lookup NU author(s): Dr Priscilla Carrillo-Barragan, Dr Jan DolfingORCiD, Dr Paul Sallis, Professor Neil GrayORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Mixed Culture Fermentation is a promising route for bioethanol production from organic wastes. Yet, achieving a stable ethanologenic activity in undefined mixed cultures remains a challenge. This work aimed to retain ethanol production from organic municipal solid waste by microbial communities enriched from sheep rumen and anaerobic sludge mixtures, under low process control (initially aerobic conditions and initial pH ≤ 5.5). To find a stable operating window , sequential inoculum transfer intervals were evaluated (14 and 3-days). Soluble fermentation product profiles and changes in the prokaryotic communities were monitored. The originally inoculated batches always produced high ethanol concentrations (60 mM; 0.070 LEtOH/Kgwaste), equivalent to 1/6 of the current corn grain-based ethanol industrial production process. Fermentative activity and community richness significantly decreased in both transfer times regimes tested. However, the 3-day transfer interval led to a stable community which consistently produced ethanol (30 mM) as its main soluble fermentation product. Originally inoculated and 3-day transferred communities consistently enriched for a solventogenic Clostridium and an acid-tolerant Pseudomonas species. Ethanologenesis,as a dominant catabolic process, is an inherent property of these mixed culture fermentations, and its maintenance across successive transfers is critically dependant on the inoculum transfer time.
Author(s): Carrillo-Barragan P, Dolfing J, Sallis P, Gray N
Publication type: Article
Publication status: Published
Journal: Biochemical Engineering Journal
Year: 2021
Volume: 166
Print publication date: 01/02/2021
Online publication date: 28/11/2020
Acceptance date: 23/11/2020
Date deposited: 18/07/2021
ISSN (print): 1369-703X
ISSN (electronic): 1873-295X
Publisher: Elsevier
URL: https://doi.org/10.1016/j.bej.2020.107875
DOI: 10.1016/j.bej.2020.107875
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