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Electro-polymerized receptor coatings for the quantitative detection of histamine with a catheter-based, diagnostic sensor

Lookup NU author(s): Dr Marloes PeetersORCiD

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This is the authors' accepted manuscript of an article that has been published in its final definitive form by American Chemical Society, 2021.

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Abstract

In this article, we report on the development of a catheter-based, biomimetic sensor as a step towards a minimally-invasive diagnostic instrument in the context of functional bowel disorders. Histamine is a key mediator in allergic and inflammatory processes in the small intestines; however, it is a challenge to determine histamine levels at the duodenal mucosa and classical bioreceptors are unsuitable for use in the digestive medium of bowel fluid. Therefore, we have developed molecularly-imprinted polypyrrole coatings for impedimetric sensing electrodes, which enable the quantification of histamine in non-diluted, human bowel fluid in a broad concentration range from 25 nM to 1 μM. The electrodes show negligible cross-sensitivity to histidine as a competitor molecule and, for comparison, we also evaluated the response of non-imprinted- and taurine-imprinted polypyrrole to histamine. Furthermore, using equivalent-circuit modelling, we found that the molecular recognition of histamine by polypyrrole primarily increases the resistive component of the electrode-liquid interface while capacitive effects are negligible. The sensor, integrated into a catheter, measures differentially to correct for non-specific adsorption effects in the complex matrix of bowel fluids and a single triggering frequency is sufficient to determine histamine concentrations. Together, these features are beneficial for real-time diagnostic tests.


Publication metadata

Author(s): Wackers G, Cornelis P, Putzeys T, Peeters M, Tack J, Troost F, Doll T, Verhaert N, Wagner P

Publication type: Article

Publication status: Published

Journal: ACS Sensors

Year: 2021

Volume: 6

Issue: 1

Pages: 100-110

Print publication date: 22/01/2021

Online publication date: 18/12/2020

Acceptance date: 02/11/2020

Date deposited: 01/12/2020

ISSN (electronic): 2379-3694

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acssensors.0c01844

DOI: 10.1021/acssensors.0c01844


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