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An in vitro human assay for evaluating immunogenic and sensitization potential of a personal care and cosmetic product

Lookup NU author(s): Dr Shaheda Ahmed, Professor Anne Dickinson

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Abstract

© 2021 Informa UK Limited, trading as Taylor & Francis Group. With the reduction or elimination of animal testing, manufacturers are left with limited options, as few robust in vitro tests are available and human studies are costly. Recently, concerns have been raised regarding potential adverse health effects associated with use of WEN by Chaz Dean (WCD) cleansing conditioners. The purpose of this study was to evaluate the immunogenic potential of a WCD hair cleansing conditioner by utilizing a novel in vitro human skin explant test. Peripheral blood mononuclear cells (PBMCs) and human skin biopsies were obtained from healthy volunteers. Monocyte derived dendritic cells (MoDCs) were generated, primed by 0.01% WCD cleansing conditioner exposure for 24 h, co-cultured with autologous lymphocytes for 4 days, and then cultured with skin biopsies for 3 days. The skin biopsies then underwent histopathological evaluation, and T cell proliferation and IFNγ levels were determined. Overall, this study showed that treatment with 0.01% WCD cleansing conditioner resulted in a negative prediction for in vivo immune response. Further, this analysis shows that the skin explant test is a viable alternative to animal testing for complex mixtures or commercially available products.


Publication metadata

Author(s): Monnot AD, Towle KM, Ahmed SS, Dickinson AM, Fung ES

Publication type: Article

Publication status: Published

Journal: Toxicology Mechanisms and Methods

Year: 2021

Volume: 31

Issue: 3

Pages: 205-211

Online publication date: 28/12/2020

Acceptance date: 20/12/2020

ISSN (print): 1537-6516

ISSN (electronic): 1537-6524

Publisher: Taylor and Francis Ltd

URL: https://doi.org/10.1080/15376516.2020.1868640

DOI: 10.1080/15376516.2020.1868640

PubMed id: 33371753


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