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Lookup NU author(s): Dr Shaheda Ahmed,
Professor Anne Dickinson
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© 2021 Informa UK Limited, trading as Taylor & Francis Group. With the reduction or elimination of animal testing, manufacturers are left with limited options, as few robust in vitro tests are available and human studies are costly. Recently, concerns have been raised regarding potential adverse health effects associated with use of WEN by Chaz Dean (WCD) cleansing conditioners. The purpose of this study was to evaluate the immunogenic potential of a WCD hair cleansing conditioner by utilizing a novel in vitro human skin explant test. Peripheral blood mononuclear cells (PBMCs) and human skin biopsies were obtained from healthy volunteers. Monocyte derived dendritic cells (MoDCs) were generated, primed by 0.01% WCD cleansing conditioner exposure for 24 h, co-cultured with autologous lymphocytes for 4 days, and then cultured with skin biopsies for 3 days. The skin biopsies then underwent histopathological evaluation, and T cell proliferation and IFNγ levels were determined. Overall, this study showed that treatment with 0.01% WCD cleansing conditioner resulted in a negative prediction for in vivo immune response. Further, this analysis shows that the skin explant test is a viable alternative to animal testing for complex mixtures or commercially available products.
Author(s): Monnot AD, Towle KM, Ahmed SS, Dickinson AM, Fung ES
Publication type: Article
Publication status: Published
Journal: Toxicology Mechanisms and Methods
Online publication date: 28/12/2020
Acceptance date: 20/12/2020
ISSN (print): 1537-6516
ISSN (electronic): 1537-6524
Publisher: Taylor and Francis Ltd
PubMed id: 33371753
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