Browse by author
Lookup NU author(s): Dr Hoang NguyenORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Reactivity of the glycosidic bonds in oat β-glucan in homogeneous acid catalyzed hydrolysis in aqueous solution was found to depend on their position in the polysaccharide chain. The bonds near the chain ends have lower hydrolytic stability in presence of HCl than the bonds in the middle. Reaction kinetics was monitored by measuring the molar mass distribution (DP ~ 30…3500) and formation of short oligosaccharides (DP < 6) under mild conditions (T = 50 °C…80 °C, cHCl = 0.05…0.25 M). Accuracy of the molar mass determination using analytical scale size-exclusion chromatography and multi-angle laser light scattering was improved by carrying out preparative scale size-exclusion separation as a pretreatment. It was found that monitoring the short-chain degradation products in addition to the molar mass distribution provides valuable information about the hydrolysis rate. The data were successfully correlated with a kinetic model that takes into account the non-random structure of oat β-glucan. The rate of cleavage of the terminal glycosidic bonds was found to be almost three times higher than at a distance of 20 or more glucose units from the chain end. The β-(1,3) bonds in oat β-glucan were found less stable against acid catalyzed hydrolysis than the β-(1,4) bonds.
Author(s): Nguyen HSH, Heinonen J, Laatikainen M, Sainio T
Publication type: Article
Publication status: Published
Journal: Chemical Engineering Journal
Year: 2020
Volume: 382
Print publication date: 15/02/2020
Online publication date: 19/09/2019
Acceptance date: 16/09/2019
ISSN (print): 1385-8947
ISSN (electronic): 1873-3212
Publisher: Elsevier
URL: https://doi.org/10.1016/j.cej.2019.122863
DOI: 10.1016/j.cej.2019.122863
Altmetrics provided by Altmetric
