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Evolution of the molar mass distribution of oat β-glucan during acid catalyzed hydrolysis in aqueous solution

Lookup NU author(s): Dr Hoang NguyenORCiD


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Reactivity of the glycosidic bonds in oat β-glucan in homogeneous acid catalyzed hydrolysis in aqueous solution was found to depend on their position in the polysaccharide chain. The bonds near the chain ends have lower hydrolytic stability in presence of HCl than the bonds in the middle. Reaction kinetics was monitored by measuring the molar mass distribution (DP ~ 30…3500) and formation of short oligosaccharides (DP < 6) under mild conditions (T = 50 °C…80 °C, cHCl = 0.05…0.25 M). Accuracy of the molar mass determination using analytical scale size-exclusion chromatography and multi-angle laser light scattering was improved by carrying out preparative scale size-exclusion separation as a pretreatment. It was found that monitoring the short-chain degradation products in addition to the molar mass distribution provides valuable information about the hydrolysis rate. The data were successfully correlated with a kinetic model that takes into account the non-random structure of oat β-glucan. The rate of cleavage of the terminal glycosidic bonds was found to be almost three times higher than at a distance of 20 or more glucose units from the chain end. The β-(1,3) bonds in oat β-glucan were found less stable against acid catalyzed hydrolysis than the β-(1,4) bonds.

Publication metadata

Author(s): Nguyen HSH, Heinonen J, Laatikainen M, Sainio T

Publication type: Article

Publication status: Published

Journal: Chemical Engineering Journal

Year: 2020

Volume: 382

Print publication date: 15/02/2020

Online publication date: 19/09/2019

Acceptance date: 16/09/2019

ISSN (print): 1385-8947

ISSN (electronic): 1873-3212

Publisher: Elsevier


DOI: 10.1016/j.cej.2019.122863


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