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Lookup NU author(s): Dr Otti CrozeORCiD
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The motility of microalgae has been studied extensively, particularly in model microorganisms such as Chlamydomonas reinhardtii. For this and other microalgal species, diurnal cycles are well known to control the metabolism, growth, and cell division. Diurnal variations, however, have been largely neglected in quantitative studies of motility. Here, we demonstrate using tracking microscopy how the motility statistics of C. reinhardtii are modulated by diurnal cycles. With nine independently inoculated cultures synchronized to the light-dark cycle at the exponential growth phase, we repeatedly observed that the mean swimming speed is greater during the dark period of a diurnal cycle. From this measurement, using a hydrodynamic power balance, we infer the mean flagellar beat frequency and conjecture that its diurnal variation reflects modulation of intracellular ATP. Our measurements also quantify the diurnal variations of the orientational and gravitactic transport of C. reinhardtii. We use this to explore the population-level consequences of diurnal variations of motility statistics by evaluating a prediction for how the gravitactic steady state changes with time during a diurnal cycle. Finally, we discuss the consequences of diurnal variations of microalgal motility in soil and pelagic environments.
Author(s): Jin D, Kotar J, Silvester E, Leptos K, Croze OA
Publication type: Article
Publication status: Published
Journal: Biophysical Journal
Year: 2020
Volume: 119
Issue: 10
Pages: 2055-2062
Print publication date: 17/11/2020
Online publication date: 19/10/2020
Acceptance date: 07/10/2020
ISSN (print): 0006-3495
ISSN (electronic): 1542-0086
Publisher: Cell Press
URL: https://doi.org/10.1016/j.bpj.2020.10.006
DOI: 10.1016/j.bpj.2020.10.006
PubMed id: 33091375
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