Toggle Main Menu Toggle Search

Open Access padlockePrints

Transgenic inhibition of interleukin-6 trans-signaling does not prevent skeletal pathologies in mucolipidosis type II mice

Lookup NU author(s): Dr Jamie Soul



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2021, The Author(s). Severe skeletal alterations are common symptoms in patients with mucolipidosis type II (MLII), a rare lysosomal storage disorder of childhood. We have previously reported that progressive bone loss in a mouse model for MLII is caused by an increased number of bone-resorbing osteoclasts, which is accompanied by elevated expression of the cytokine interleukin-6 (IL-6) in the bone microenvironment. In the present study we addressed the question, if pharmacological blockade of IL-6 can prevent the low bone mass phenotype of MLII mice. Since the cellular IL-6 response can be mediated by either the membrane-bound (classic signaling) or the soluble IL-6 receptor (trans-signaling), we first performed cell culture assays and found that both pathways can increase osteoclastogenesis. We then crossed MLII mice with transgenic mice expressing the recombinant soluble fusion protein sgp130Fc, which represents a natural inhibitor of IL-6 trans-signaling. By undecalcified histology and bone-specific histomorphometry we found that high circulating sgp130Fc levels do not affect skeletal growth or remodeling in wild-type mice. Most importantly, blockade of IL-6 trans-signaling did neither reduce osteoclastogenesis, nor increase bone mass in MLII mice. Therefore, our data clearly demonstrate that the bone phenotype of MLII mice cannot be corrected by blocking the IL-6 trans-signaling.

Publication metadata

Author(s): Westermann LM, Baranowsky A, Di Lorenzo G, Danyukova T, Soul J, Schwartz J-M, Hendrickx G, Amling M, Rose-John S, Garbers C, Schinke T, Pohl S

Publication type: Article

Publication status: Published

Journal: Scientific Reports

Year: 2021

Volume: 11

Online publication date: 11/02/2021

Acceptance date: 25/01/2021

Date deposited: 08/04/2021

ISSN (electronic): 2045-2322

Publisher: Nature Research


DOI: 10.1038/s41598-021-82802-3


Altmetrics provided by Altmetric


Funder referenceFunder name
Projekt DEAL