Browse by author
Lookup NU author(s): Mahdi Lamb, Dr Lisa Ferguson, Chris Lodge, Professor Mary Herbert
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2021 The Authors. Published under the terms of the CC BY 4.0 licenseGenome haploidization involves sequential loss of cohesin from chromosome arms and centromeres during two meiotic divisions. At centromeres, cohesin's Rec8 subunit is protected from separase cleavage at meiosis I and then deprotected to allow its cleavage at meiosis II. Protection of centromeric cohesin by shugoshin-PP2A seems evolutionarily conserved. However, deprotection has been proposed to rely on spindle forces separating the Rec8 protector from cohesin at metaphase II in mammalian oocytes and on APC/C-dependent destruction of the protector at anaphase II in yeast. Here, we have activated APC/C in the absence of sister kinetochore biorientation at meiosis II in yeast and mouse oocytes, and find that bipolar spindle forces are dispensable for sister centromere separation in both systems. Furthermore, we show that at least in yeast, protection of Rec8 by shugoshin and inhibition of separase by securin are both required for the stability of centromeric cohesin at metaphase II. Our data imply that related mechanisms preserve the integrity of dyad chromosomes during the short metaphase II of yeast and the prolonged metaphase II arrest of mammalian oocytes.
Author(s): Mengoli V, Jonak K, Lyzak O, Lamb M, Lister LM, Lodge C, Rojas J, Zagoriy I, Herbert M, Zachariae W
Publication type: Article
Publication status: Published
Journal: EMBO Journal
Year: 2021
Online publication date: 01/03/2021
Acceptance date: 22/01/2021
Date deposited: 19/03/2021
ISSN (print): 0261-4189
ISSN (electronic): 1460-2075
Publisher: Wiley-VCH Verlag
URL: https://doi.org/10.15252/embj.2020106812
DOI: 10.15252/embj.2020106812
Altmetrics provided by Altmetric
