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Lookup NU author(s): Emeritus Professor Hans-Peter Klenk
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© 2021, Analele Universitatii din Oradea, Fascicula Biologie. All rights reserved.Actinobacteria originated from extreme ecosystems remains the most interesting source of new antibiotics. An actinobacterial strain, named AHO23, was isolated from Ahaggar region soil in Algeria, using the dilution agar plating method with chitin-vitamins B medium supplemented with nalidixic acid and actidione. The morphological characteristics showed that strain AHO23 belonged to the genus Saccharothrix. Analysis of the 16S rRNA gene sequence of this strain AH023 showed 98.95% of similarity with the closely related species Saccharothrix xinjiangensis NBRC 101911T. AH023 exhibited a strong activity against Gram-negative bacteria (15–36 mm), as well as good activity against Gram-positive bacteria (29–34 mm). Candida albicans (ATCC10237) was also inhibited (20 mm). Moreover, AH023 presented a strong activity against mycotoxigenic and phytopathogenic fungi (21–36 mm), with the highest activity (36 mm) against Fusarium culmorum. Bioactive metabolites from AH023 culture on ISP2 medium were extracted using four organics solvents: n-hexane, dichloromethane, ethyl-acetate and n-butanol. The highest antibacterial activities were detected in the n-butanol extract.
Author(s): Saadi SA, Meklat A, Mokrane S, Yaiche Achour H, Holtz MD, Klenk H-P, Bouras N
Publication type: Article
Publication status: Published
Journal: Analele Universitatii din Oradea, Fascicula Biologie
Year: 2021
Volume: 28
Issue: 1
Pages: 71-77
Online publication date: 01/03/2021
Acceptance date: 26/02/2021
ISSN (print): 1224-5119
ISSN (electronic): 1844-7589
Publisher: Universitatea din Oradea
URL: https://www.bioresearch.ro/2021-1/071-077-AUOFB.28.1.2021-SAADI.S.A-Isolation.and.characterization.pdf
