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Bioprospecting of wild type ethanologenic yeast for ethanol fuel production from wastewater-grown microalgae

Lookup NU author(s): Dr Sharon Velasquez Orta


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© 2021, The Author(s).Background: Wild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. In this study, wild-type ethanologenic yeasts isolated from a secondary effluent were assessed for their capability to ferment saccharified microalgae sugars. Results: Yeast species in wastewater were identified sequencing the Internal Transcribed Spacers 1 and 2 regions of the ribosomal cluster. Concurrently, microalgae biomass sugars were saccharified via acid hydrolysis, producing 5.0 ± 0.3 g L−1 of fermentable sugars. Glucose consumption and ethanol production of yeasts in hydrolyzed-microalgae liquor were tested at different initial sugar concentrations and fermentation time. The predominant ethanologenic yeast species was identified as Candida sp., and glucose consumption for this strain and S. cerevisiae achieved 75% and 87% of the initial concentration at optimal conditions, respectively. Relatively similar ethanol yields were determined for both species, achieving 0.45 ± 0.05 (S. cerevisiae) and 0.46 ± 0.05 g ethanol per g glucose (Candida sp.). Conclusion: Overall, the results provide a first insight of the fermentation capacities of specific wild-type Candida species, and their potential role in ethanol industries seeking to improve their cost-efficiency. [Figure not available: see fulltext.]

Publication metadata

Author(s): Romero-Frasca E, Velasquez-Orta SB, Escobar-Sanchez V, Tinoco-Valencia R, Orta Ledesma MT

Publication type: Article

Publication status: Published

Journal: Biotechnology for Biofuels

Year: 2021

Volume: 14

Issue: 1

Online publication date: 09/04/2021

Acceptance date: 07/03/2021

Date deposited: 26/04/2021

ISSN (print): 1754-6834

ISSN (electronic): 1754-6834

Publisher: BioMed Central Ltd


DOI: 10.1186/s13068-021-01925-x


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