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Blobs and curves: Object-based colocalisation for plant cells

Lookup NU author(s): Professor Boguslaw ObaraORCiD

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Abstract

© CSIRO 2015. Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.

Publication metadata

Author(s): Nelson CJ, Duckney P, Hawkins TJ, Deeks MJ, Laissue PP, Hussey PJ, Obara B

Publication type: Article

Publication status: Published

Journal: Functional Plant Biology

Year: 2015

Volume: 42

Issue: 5

Pages: 471-485

Print publication date: 01/05/2015

Online publication date: 22/09/2014

Acceptance date: 21/07/2014

ISSN (print): 1445-4408

ISSN (electronic): 1445-4416

Publisher: CSIRO

URL: https://doi.org/10.1071/FP14047

DOI: 10.1071/FP14047

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