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Lookup NU author(s): Professor Boguslaw Obara
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© CSIRO 2015. Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.
Author(s): Nelson CJ, Duckney P, Hawkins TJ, Deeks MJ, Laissue PP, Hussey PJ, Obara B
Publication type: Article
Publication status: Published
Journal: Functional Plant Biology
Print publication date: 01/05/2015
Online publication date: 22/09/2014
Acceptance date: 21/07/2014
ISSN (print): 1445-4408
ISSN (electronic): 1445-4416
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