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Lookup NU author(s): Dr Yuko Takeda, Dr Magomet AushevORCiD, Dr Meenakshi Choudhary, Professor Mary Herbert
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
© 2021 The AuthorsMost human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic divisions of the embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at the pronuclear interface are thought to be more likely to develop into healthy euploid embryos. Here, we show that the parental genomes cluster with nucleoli in each pronucleus within human and bovine zygotes, and clustering is required for the reliable unification of the parental genomes after fertilization. During migration of intact pronuclei, the parental genomes polarize toward each other in a process driven by centrosomes, dynein, microtubules, and nuclear pore complexes. The maternal and paternal chromosomes eventually cluster at the pronuclear interface, in direct proximity to each other, yet separated. Parental genome clustering ensures the rapid unification of the parental genomes on nuclear envelope breakdown. However, clustering often fails, leading to chromosome segregation errors and micronuclei, incompatible with healthy embryo development.
Author(s): Cavazza T, Takeda Y, Politi AZ, Aushev M, Aldag P, Baker C, Choudhary M, Bucevicius J, Lukinavicius G, Elder K, Blayney M, Lucas-Hahn A, Niemann H, Herbert M, Schuh M
Publication type: Article
Publication status: Published
Journal: Cell
Year: 2021
Volume: 184
Issue: 11
Pages: 2860-2877.e22
Online publication date: 07/05/2021
Acceptance date: 08/04/2021
Date deposited: 11/06/2021
ISSN (print): 0092-8674
ISSN (electronic): 1097-4172
Publisher: Elsevier B.V.
URL: https://doi.org/10.1016/j.cell.2021.04.013
DOI: 10.1016/j.cell.2021.04.013
PubMed id: 33964210
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