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Single-Cell Approaches for Studying the Role of Mitochondrial DNA in Neurodegenerative Disease

Lookup NU author(s): Dr Joanna Elson


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© 2021, Springer Science+Business Media, LLC, part of Springer Nature.In light of accumulating evidence suggestive of cell type-specific vulnerabilities as a result of normal aging processes that adversely affect the brain, as well as age-related neurodegenerative disorders such as Parkinson’s disease (PD), the current chapter highlights how we study mitochondrial DNA (mtDNA) changes at a single-cell level. In particular, we comment on increasing questioning of the narrow neurocentric view of such pathologies, where microglia and astrocytes have traditionally been considered bystanders rather than players in related pathological processes. Here we review the contribution made by single-cell mtDNA alterations towards neuronal vulnerability seen in neurodegenerative disorders, focusing on PD as a prominent example. In addition, we give an overview of methodologies that support such experimental investigations. In considering the significant advances that have been made in recent times for developing mitochondria-specific therapies, investigations to account for cell type-specific mitochondrial patterns and how these are altered by disease hold promise for delivering more effective disease-modifying therapeutics.

Publication metadata

Author(s): Bailey LJ, Elson JL, Pienaar IS

Publication type: Book Chapter

Publication status: Published

Book Title: Methods in Molecular Biology

Year: 2021

Volume: 2277

Pages: 299-329

Online publication date: 03/06/2021

Acceptance date: 02/04/2018

Publisher: Humana Press Inc.

Place Published: Humana, New York


DOI: 10.1007/978-1-0716-1270-5_19

PubMed id: 34080159

Library holdings: Search Newcastle University Library for this item

ISBN: 9781071612699