The Isolation and Deep Sequencing of Mitochondrial DNA

Bury, AG; Robertson, FM; Pyle, A; Payne, BAI; Hudson, G

doi:10.1007/978-1-0716-1270-5_27

The Isolation and Deep Sequencing of Mitochondrial DNA

Lookup NU author(s): Alex Bury, Dr Fiona Robertson, Dr Angela Pyle, Dr Brendan Payne ORCiD, Professor Gavin Hudson

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Abstract

© 2021, Springer Science+Business Media, LLC, part of Springer Nature.In recent years, next-generation sequencing (NGS) has become a powerful tool for studying both inherited and somatic heteroplasmic mitochondrial DNA (mtDNA) variation. NGS has proved particularly powerful when combined with single-cell isolation techniques, allowing the investigation of low-level heteroplasmic variants both between cells and within tissues. Nevertheless, there remain significant challenges, especially around the selective enrichment of mtDNA from total cellular DNA and the avoidance of nuclear pseudogenes. This chapter summarizes the techniques needed to enrich, amplify, sequence, and analyse mtDNA using NGS.