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Lookup NU author(s): Lynne Overman, Dr Steven LisgoORCiD
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© 2021, The Author(s), under exclusive licence to Springer Nature America, Inc.The human neonatal cerebellum is one-fourth of its adult size yet contains the blueprint required to integrate environmental cues with developing motor, cognitive and emotional skills into adulthood. Although mature cerebellar neuroanatomy is well studied, understanding of its developmental origins is limited. In this study, we systematically mapped the molecular, cellular and spatial composition of human fetal cerebellum by combining laser capture microscopy and SPLiT-seq single-nucleus transcriptomics. We profiled functionally distinct regions and gene expression dynamics within cell types and across development. The resulting cell atlas demonstrates that the molecular organization of the cerebellar anlage recapitulates cytoarchitecturally distinct regions and developmentally transient cell types that are distinct from the mouse cerebellum. By mapping genes dominant for pediatric and adult neurological disorders onto our dataset, we identify relevant cell types underlying disease mechanisms. These data provide a resource for probing the cellular basis of human cerebellar development and disease.
Author(s): Aldinger KA, Thomson Z, Phelps IG, Haldipur P, Deng M, Timms AE, Hirano M, Santpere G, Roco C, Rosenberg AB, Lorente-Galdos B, Gulden FO, O'Day D, Overman LM, Lisgo SN, Alexandre P, Sestan N, Doherty D, Dobyns WB, Seelig G, Glass IA, Millen KJ
Publication type: Article
Publication status: Published
Journal: Nature Neuroscience
Year: 2021
Volume: 24
Pages: 1163–1175
Online publication date: 17/06/2021
Acceptance date: 10/05/2021
ISSN (print): 1097-6256
ISSN (electronic): 1546-1726
Publisher: Nature Research
URL: https://doi.org/10.1038/s41593-021-00872-y
DOI: 10.1038/s41593-021-00872-y
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