Toggle Main Menu Toggle Search

Open Access padlockePrints

Correlative single-molecule fluorescence barcoding of gene regulation in Saccharomyces cerevisiae

Lookup NU author(s): Dr Adam WollmanORCiD



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2020 The Authors. Most cells adapt to their environment by switching combinations of genes on and off through a complex interplay of transcription factor proteins (TFs). The mechanisms by which TFs respond to signals, move into the nucleus and find specific binding sites in target genes is still largely unknown. Single-molecule fluorescence microscopes, which can image single TFs in live cells, have begun to elucidate the problem. Here, we show that different environmental signals, in this case carbon sources, yield a unique single-molecule fluorescence pattern of foci of a key metabolic regulating transcription factor, Mig1, in the nucleus of the budding yeast, Saccharomyces cerevisiae. This pattern serves as a ‘barcode’ of the gene regulatory state of the cells which can be correlated with cell growth characteristics and other biological function.

Publication metadata

Author(s): Shashkova S, Nystrom T, Leake MC, Wollman AJM

Publication type: Article

Publication status: Published

Journal: Methods

Year: 2021

Volume: 193

Pages: 62-67

Print publication date: 01/09/2021

Online publication date: 18/10/2020

Acceptance date: 15/10/2020

Date deposited: 26/07/2021

ISSN (print): 1046-2023

ISSN (electronic): 1095-9130

Publisher: Academic Press Inc.


DOI: 10.1016/j.ymeth.2020.10.009

PubMed id: 33086048


Altmetrics provided by Altmetric


Funder referenceFunder name