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Lookup NU author(s): Dr Adam WollmanORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2020 The Authors. Most cells adapt to their environment by switching combinations of genes on and off through a complex interplay of transcription factor proteins (TFs). The mechanisms by which TFs respond to signals, move into the nucleus and find specific binding sites in target genes is still largely unknown. Single-molecule fluorescence microscopes, which can image single TFs in live cells, have begun to elucidate the problem. Here, we show that different environmental signals, in this case carbon sources, yield a unique single-molecule fluorescence pattern of foci of a key metabolic regulating transcription factor, Mig1, in the nucleus of the budding yeast, Saccharomyces cerevisiae. This pattern serves as a ‘barcode’ of the gene regulatory state of the cells which can be correlated with cell growth characteristics and other biological function.
Author(s): Shashkova S, Nystrom T, Leake MC, Wollman AJM
Publication type: Article
Publication status: Published
Journal: Methods
Year: 2021
Volume: 193
Pages: 62-67
Print publication date: 01/09/2021
Online publication date: 18/10/2020
Acceptance date: 15/10/2020
Date deposited: 26/07/2021
ISSN (print): 1046-2023
ISSN (electronic): 1095-9130
Publisher: Academic Press Inc.
URL: https://doi.org/10.1016/j.ymeth.2020.10.009
DOI: 10.1016/j.ymeth.2020.10.009
PubMed id: 33086048
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