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The Aurora B gradient sustains kinetochore stability in anaphase

Lookup NU author(s): Dr Diana Papini, Dr Mark Levasseur, Professor Jonathan Higgins

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Kinetochores assemble on chromosomes in mitosis to allow microtubules to attach and bring about accurate chromosome segregation. The kinases Cyclin B-Cdk1 and Aurora B are crucial for the formation of stable kinetochores. However, the activity of these two kinases appears to decline dramatically at centromeres during anaphase onset, precisely when microtubule attachments are required to move chromosomes towards opposite poles of the dividing cell. We find that, although Aurora B leaves centromeres at anaphase, a gradient of Aurora B activity centred on the central spindle is still able to phosphorylate kinetochore substrates such as Dsn1 to modulate kinetochore stability in anaphase and to regulate kinetochore disassembly as cells enter telophase. We provide a model to explain how Aurora B co-operates with Cyclin B-Cdk1 to maintain kinetochore function in anaphase.


Publication metadata

Author(s): Papini D, Levasseur MD, Higgins JMG

Publication type: Article

Publication status: Published

Journal: Cell Reports

Year: 2021

Volume: 37

Issue: 6

Print publication date: 09/11/2021

Online publication date: 09/11/2021

Acceptance date: 17/09/2021

Date deposited: 12/11/2021

ISSN (electronic): 2211-1247

Publisher: Cell Press

URL: https://doi.org/10.1016/j.celrep.2021.109818

DOI: 10.1016/j.celrep.2021.109818


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