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Assessment of nematodes in Punjab Urial (Ovis vignei punjabiensis) population in Kalabagh Game Reserve: development of a DNA barcode approach

Lookup NU author(s): Dr Jordan CuffORCiD


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Punjab urial (Ovis vignei punjabiensis) is a wild sheep of Pakistan, considered a vulnerable species by IUCN. Major threats to urial populations include habitat loss and poaching, causing severe declines in its population. Nematode infections may also compromise urial survival, but little is known about Punjab urial gastrointestinal nematodes. In this study, a novel DNA barcoding approach was developed using ITS-I as a target region, with a primer pair designed to amplify frequently reported nematode species for small ruminants. The novel primer pair was validated in silico and in vitro and subsequently used to determine the presence of nematodes in Punjab urial samples from Kala Bagh Game Reserve, District Mianwali (Pakistan). DNA barcoding revealed a higher prevalence of Haemonchus contortus (73.91%), Trichuris ovis (16.30%) and Trichostrongylus axei (3.26%) in Punjab urial. This study demonstrates that the novel DNA barcoding approach is a robust tool to detect nematode parasites from faecal samples of Punjab urial. This method can be used to detect nematode infections in wild and domestic hosts for surveillance and population conservation.

Publication metadata

Author(s): Bajwa AA, Cuff JP, Imran M, Islam S, Mansha R, Ashraf K, Khan A, Rashid MI, Zahoor MY, Khan WA, Rehman HU, Nadeem A, Orozco-terWengel P, Shehzad W

Publication type: Article

Publication status: Published

Journal: European Journal of Wildlife Research

Year: 2019

Volume: 65

Online publication date: 13/07/2019

Acceptance date: 01/07/2019

ISSN (print): 1612-4642

ISSN (electronic): 1439-0574

Publisher: Springer Nature


DOI: 10.1007/s10344-019-1298-y


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