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Lookup NU author(s): Dr Patrycja Golinska, Professor Michael Goodfellow
This is the authors' accepted manuscript of an article that has been published in its final definitive form by Microbiology Society, 2021.
For re-use rights please refer to the publisher's terms and conditions.
© 2021 The Authors. The taxonomic status of two filamentous actinobacteria, isolates NF23 and NL8T, recovered from the litter layer of a pine forest soil in Poland was established in a genome-based polyphasic study. The isolates showed a combination of chemotaxonomic, morphological and physiological properties associated with their classification in the genus Catenulispora. They formed a well supported lineage within the Catenulispora 16S rRNA gene tree and were most closely related to the type strains of Catenulispora acidiphila (99.1%), Catenulispora pinisilvae (99.9%) and Catenulispora rubra (99.1%), and like them, were found to have large genomes (10.8 and 11.5 Mbp, respectively). A phylogenomic tree based on the draft genomes of isolates NF23 and NL8T and their phylogenetic neighbours showed that they formed a distinct branch in the Catenulispora clade that was most closely related to C. pinisilvae DSM 111109T. The isolates shared a combination of genomic, genotypic and phenotypic features, and had high average nucleotide index (ANI) and digital DNA:DNA hybridization (dDDH) similarities consistent with their assignment to the same species. The isolates were distinguished from the C. acidiphila, C. pinisilvae and C. rubra strains by a wealth of taxonomic data and by low ANI (84.9–93.9%) and dDDH (29.6–54.7%) values. It is proposed that the isolates be classified in the genus Catenulispora as C. pinistramenti sp. nov. with isolate NL8T (=DSM 111110T=PCM 3045T) as the type strain. The genomes of strains NF23 and NL8T are rich in natural product-biosynthetic gene clusters hence these strains have the potential to synthesize new specialised metabolites.
Author(s): Swiecimska M, Golinska P, Wypij M, Goodfellow M
Publication type: Article
Publication status: Published
Journal: International Journal of Systematic and Evolutionary Microbiology
Year: 2021
Volume: 71
Issue: 10
Online publication date: 21/10/2021
Acceptance date: 06/09/2021
Date deposited: 20/12/2021
ISSN (print): 1466-5026
ISSN (electronic): 1466-5034
Publisher: Microbiology Society
URL: https://doi.org/10.1099/ijsem.0.005063
DOI: 10.1099/ijsem.0.005063
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