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Lookup NU author(s): Dr Miguel Garcia Dos Santos XavierORCiD
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
Paternal exposure to environmental stressors elicits distinct changes to the sperm sncRNA profile, modifications that have significant post-fertilization consequences. Despite this knowledge, there remains limited mechanistic understanding of how paternal exposures modify the sperm sncRNA landscape. Here, we report the acute sensitivity of the sperm sncRNA profile to the reproductive toxicant acrylamide. Furthermore, we trace the differential accumulation of acrylamide-responsive sncRNAs to coincide with sperm transit of the proximal (caput) segment of the epididymis, wherein acrylamide exposure alters the abundance of several transcription factors implicated in the expression of acrylamide-sensitive sncRNAs. We also identify extracellular vesicles secreted from the caput epithelium in relaying altered sncRNA profiles to maturing spermatozoa and dysregulated gene expression during early embryonic development following fertilization by acrylamide-exposed spermatozoa. These data provide mechanistic links to account for how environmental insults can alter the sperm epigenome and compromise the transcriptomic profile of early embryos.
Author(s): Trigg NA, Skerrett-Byrne DA, Xavier MJ, Zhou W, Anderson AL, Stanger SJ, Katen AL, De Iuliis GN, Dun MD, Roman SD, Eamens AL, Nixon B
Publication type: Article
Publication status: Published
Journal: Cell Reports
Year: 2021
Volume: 37
Issue: 1
Pages: 109787
Print publication date: 05/10/2021
Online publication date: 05/10/2021
Acceptance date: 09/09/2021
Date deposited: 22/08/2023
ISSN (electronic): 2211-1247
Publisher: Elsevier
URL: https://doi.org/10.1016/j.celrep.2021.109787
DOI: 10.1016/j.celrep.2021.109787
Data Access Statement: The data discussed in this publication have been deposited in the respective repositories. RNA-seq and ncRNA-seq data have been deposited in NCBI’s Gene Expression Omnibus and are accessible as of the day of publication. Accession numbers are listed in the Key resources table. Mass spectrometry data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE repository (PXD022865) (Perez-Riverol et al., 2019) for discovery TMT data or via Panorama Public (Sharma et al., 2018b) (https://panoramaweb.org/yjjOG1.url) for targeted PRM data. Accession numbers are listed in the Key resources table. This paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
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