Browse by author
Lookup NU author(s): Dr Sabrina MackinnonORCiD
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
This project provides the tools and data to develop small molecule inhibitors for an inherited metabolic disorder (Primary hyperoxaluria type 1) due to the defective enzyme (AGXT), by targeting the enzyme (HAO1) upstream of the glyoxylate metabolic pathway to mitigate the defect (i.e. substrate reduction approach). This TEP package includes recombinant human HAO1 purification protocols, structures of the HAO1 in different states, in vitro assays to detect ligand/inhibitor binding (DSF, SPR) and enzyme activity (amplex red assay) of human HAO1, as well as initial chemical matters identified from crystallography-based fragment screening.
Author(s): MacKinnon S, Arruda Bezerra G, Krojer T, Bradley AR, Talon R, Brandao-Neto J, Douangamath A, Oppermann U, von Delft F, Brennan PE, Yue WW
Publication type: Online Publication
Publication status: Published
Access Year: 2022
Acceptance date: 30/06/2018
Place Published: Geneva
Access Date: 8 April