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Lookup NU author(s): Dr Evelyn JensenORCiD
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© The American Genetic Association. 2018. All rights reserved.Genome-wide assessments allow for fuller characterization of genetic diversity, finer-scale population delineation, and better detection of demographically significant units to guide conservation compared with those based on “traditional” markers. Galapagos giant tortoises (Chelonoidis spp.) have long provided a case study for how evolutionary genetics may be applied to advance species conservation. Ongoing efforts to bolster tortoise populations, which have declined by 90%, have been informed by analyses of mitochondrial DNA sequence and microsatellite genotypic data, but could benefit from genome-wide markers. Taking this next step, we used double-digest restriction-site associated DNA sequencing to collect genotypic data at >26 000 single nucleotide polymorphisms (SNPs) for 117 individuals representing all recognized extant Galapagos giant tortoise species. We then quantified genetic diversity, population structure, and compared results to estimates from mitochondrial DNA and microsatellite loci. Our analyses detected 12 genetic lineages concordant with the 11 named species as well as previously described structure within one species, C. becki. Furthermore, the SNPs provided increased resolution, detecting admixture in 4 individuals. SNP-based estimates of diversity and differentiation were significantly correlated with those derived from nuclear microsatellite loci and mitochondrial DNA sequences. The SNP toolkit presented here will serve as a resource for advancing efforts to understand tortoise evolution, species radiations, and aid conservation of the Galapagos tortoise species complex.
Author(s): Miller JM, Quinzin MC, Edwards DL, Eaton DAR, Jensen EL, Russello MA, Gibbs JP, Tapia W, Rueda D, Caccone A
Publication type: Article
Publication status: Published
Journal: Journal of Heredity
Year: 2018
Volume: 109
Issue: 6
Pages: 611-619
Online publication date: 07/07/2018
Acceptance date: 04/07/2018
ISSN (print): 0022-1503
ISSN (electronic): 1465-7333
Publisher: Oxford University Press
URL: https://doi.org/10.1093/jhered/esy031
DOI: 10.1093/jhered/esy031
PubMed id: 29986032
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