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Influence of design and material charateristics on 3D printed flow-cells for heat transwer-based analytical devices

Lookup NU author(s): Dr Ollie Jamieson, Jack Reeder, Jenny Olsen, Dr Richard Law, Dr Marloes PeetersORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

Redesigning 3D-printed flow cells is reported used for heat transfer based detection of biomolecules from a flow-through system to an addition-type measurement cell. The aim of this study is to assess the performance of this new measurement design and critically analyse the influence of material properties and 3D printing approach on thermal analysis. Particular attention is paid to reduce the time to stabilisation, the sample volume in order to make the technique suitable for clinical applications, and improving the sensitivity of the platform by decreasing the noise and interference of air bubbles. The three different approaches that were studied included a filament polylactic acid cell using only fused filament fabrication (FFF), a resin cell printed using stereolitography (SLA), and finally a design made of copper, which was manufactured by combining metal injection moulding (MIM) with fused filament fabrication (FFF). Computational fluid dynamic (CFD) modelling was undertaken using ANSYS Fluent V18.1 to provide insight into the flow of heat within the measurement cell, facilitating optimisation of the system and theoretical response speed.It was shown that the measurement cells using SLA had the lowest noise (~ 0.6%) and shortest measurement time (15 min), whereas measurement cells produced using other approaches had lower specificity or suffered from voiding issues. Finally, we assessed the potential of these new designs for detection of biomolecules and amoxicillin, a commonly used beta lactam antibiotic, to demonstrate the proof of concept. It can be concluded that the resin addition-type measurement cells produced with SLA are an interesting affordable alternative, which were able to detect amoxicillin with high sensitivity and have great promise for clinical applications due to the disposable nature of the measurement cells in addition to small sample volumes.


Publication metadata

Author(s): Figueiredo L, Vieira F, Jamieson O, Reeder J, McLean T, Olsen J, Crapnell R, Whittingham M, Banks C, Law R, Gruber J, Peeters M

Publication type: Article

Publication status: Published

Journal: Microchimica Acta

Year: 2022

Volume: 189

Online publication date: 24/01/2022

Acceptance date: 26/12/2021

Date deposited: 02/02/2022

ISSN (print): 0026-3672

ISSN (electronic): 1436-5073

Publisher: Springer

URL: https://doi.org/10.1007/s00604-022-05163-2

DOI: 10.1007/s00604-022-05163-2


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Funding

Funder referenceFunder name
307501/2019-1
424027/2018-6
E21-6023112160
Seedcorn2020100303

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