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Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method

Lookup NU author(s): Professor Marloes PeetersORCiD

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).

Abstract

© 2022. Serum creatinine concentration is an important clinical measure of kidney function. However, standard methods of detection, such as the Jaffe method or enzymatic assays, suffer several disadvantages, including non-specificity and procedural complexity, or high cost, respectively. In this work, we propose the use of nano-molecularly imprinted polymers (nMIPs) in conjunction with the novel Heat Transfer Method (HTM) as a promising alternative sensing platform to these existing methods for measuring serum creatinine concentration. More specifically, it is shown that creatinine-imprinted nMIPs can be produced using a solid-phase templating method, and that simple drop-casting onto a cheap, disposable substrate can be used in conjunction with HTM to detect creatinine with a limit-of-detection of (7.0 ± 0.5) μM in buffer solutions. Furthermore, the nMIPs are shown to selectively bind creatinine in comparison to several similar molecules, and the sensing platform is demonstrated to be able to detect changes in creatinine concentration in complex blood plasma samples.

Publication metadata

Author(s): Williams RJ, Crapnell RD, Dempsey NC, Peeters M, Banks CE

Publication type: Article

Publication status: Published

Journal: Talanta Open

Year: 2022

Volume: 5

Print publication date: 01/08/2022

Online publication date: 23/01/2022

Acceptance date: 22/01/2022

Date deposited: 07/02/2022

ISSN (electronic): 2666-8319

Publisher: Elsevier BV

URL: https://doi.org/10.1016/j.talo.2022.100087

DOI: 10.1016/j.talo.2022.100087

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