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Versioning biological cells for trustworthy cell engineering

Lookup NU author(s): Jonathan Tellechea Luzardo, Leanne Hobbs, Dr Lenka Pelechova, Professor Simon WoodsORCiD, Professor Natalio KrasnogorORCiD

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


Abstract

© 2022. The Author(s). "Full-stack" biotechnology platforms for cell line (re)programming are on the horizon, thanks mostly to (a) advances in gene synthesis and editing techniques as well as (b) the growing integration of life science research with informatics, the internet of things and automation. These emerging platforms will accelerate the production and consumption of biological products. Hence, traceability, transparency, and-ultimately-trustworthiness is required from cradle to grave for engineered cell lines and their engineering processes. Here we report a cloud-based version control system for biotechnology that (a) keeps track and organizes the digital data produced during cell engineering and (b) molecularly links that data to the associated living samples. Barcoding protocols, based on standard genetic engineering methods, to molecularly link to the cloud-based version control system six species, including gram-negative and gram-positive bacteria as well as eukaryote cells, are shown. We argue that version control for cell engineering marks a significant step toward more open, reproducible, easier to trace and share, and more trustworthy engineering biology.


Publication metadata

Author(s): Tellechea-Luzardo J, Hobbs L, Velazquez E, Pelechova L, Woods S, de Lorenzo V, Krasnogor N

Publication type: Article

Publication status: Published

Journal: Nature communications

Year: 2022

Volume: 13

Issue: 1

Online publication date: 09/02/2022

Acceptance date: 21/01/2022

Date deposited: 14/03/2022

ISSN (electronic): 2041-1723

Publisher: Nature Publishing Group

URL: https://doi.org/10.1038/s41467-022-28350-4

DOI: 10.1038/s41467-022-28350-4

PubMed id: 35140226


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Funding

Funder referenceFunder name
EP/N031962/1EPSRC
H2020-BIO-CN-2019-87029
H2020-FET-OPEN-RIA-2017-1-766975
H2020-NMBP-TR-IND/H2020-NMBP-BIO-2018-814650

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