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Lookup NU author(s): Syed Gillani, Professor Jonathan HigginsORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022. Published by The Company of Biologists Ltd. PCTAIRE1 (also known as CDK16) is a serine-threonine kinase implicated in physiological processes like neuronal development, vesicle trafficking, spermatogenesis and cell proliferation. However, its exact role in cell division remains unclear. In this study, using a library screening approach, we identified PCTAIRE1 among several candidates that resisted mitotic arrest and mitotic cell death induced by polyomavirus small T (PolST) expression in mammalian cells. Our study showed that PCTAIRE1 is a mitotic kinase that localizes at centrosomes during G2 and at spindle poles as the cells enter mitosis, and then at the midbody during cytokinesis. We also report that PCTAIRE1 protein levels fluctuate through the cell cycle and reach their peak at mitosis, during which there is an increase in PCTAIRE1 phosphorylation as well. Interestingly, knockdown of PCTAIRE1 resulted in aberrant mitosis by interfering with spindle assembly and chromosome segregation. Further, we found that PCTAIRE1 promotes resistance of cancer cells to antimitotic drugs, and this underscores the significance of PCTAIRE1 as a potential drug target for overcoming chemotherapeutic resistance. Taken together, these studies establish PCTAIRE1 as a critical mediator of mitotic progression and highlight its role in chemotherapeutic resistance. This article has an associated First Person interview with the first author of the paper.
Author(s): Gillani SQ, Reshi I, Nabi N, Nisa MU, Sarwar Z, Bhat S, Roberts TM, Higgins JMG, Andrabi S
Publication type: Article
Publication status: Published
Journal: Journal of Cell Science
Year: 2022
Volume: 135
Issue: 3
Online publication date: 09/02/2022
Acceptance date: 29/12/2021
Date deposited: 10/03/2022
ISSN (print): 0021-9533
ISSN (electronic): 1477-9137
Publisher: Company of Biologists Ltd
URL: https://doi.org/10.1242/jcs.258831
DOI: 10.1242/jcs.258831
PubMed id: 35044463
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