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A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation

Lookup NU author(s): Valeria Verrone, Professor Anil Wipat

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© 2022. The Author(s). BACKGROUND: Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segments of chromosome from one cell to another between B. subtilis. RESULTS: In this study, we applied mobilization of the B. subtilis chromosome mediated by pLS20catΔoriT to transform G. kaustophilus. We constructed a gene cassette to be integrated into G. kaustophilus and designed it within the B. subtilis chromosome. The pLS20catΔoriT-mediated conjugation successfully transferred the gene cassette from the B. subtilis chromosome into the G. kaustophilus allowing for the desired genetic transformation. CONCLUSIONS: This transformation approach described here will provide a new tool to facilitate the flexible genetic manipulation of G. kaustophilus.

Publication metadata

Author(s): Mori K, Fukui K, Amatsu R, Ishikawa S, Verrone V, Wipat A, Meijer WJJ, Yoshida K-I

Publication type: Article

Publication status: Published

Journal: Microbial Cell Factories

Year: 2022

Volume: 21

Issue: 1

Online publication date: 08/03/2022

Acceptance date: 19/02/2022

Date deposited: 21/03/2022

ISSN (electronic): 1475-2859

Publisher: BioMed Central Ltd.

URL: https://doi.org/10.1186/s12934-022-01759-8

DOI: 10.1186/s12934-022-01759-8

PubMed id: 35260160

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