Browse by author
Lookup NU author(s): Valeria Verrone,
Professor Anil Wipat
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022. The Author(s). BACKGROUND: Geobacillus kaustophilus is a thermophilic Gram-positive bacterium. Methods for its transformation are still under development. Earlier studies have demonstrated that pLS20catΔoriT mobilized the resident mobile plasmids from Bacillus subtilis to G. kaustophilus and transferred long segments of chromosome from one cell to another between B. subtilis. RESULTS: In this study, we applied mobilization of the B. subtilis chromosome mediated by pLS20catΔoriT to transform G. kaustophilus. We constructed a gene cassette to be integrated into G. kaustophilus and designed it within the B. subtilis chromosome. The pLS20catΔoriT-mediated conjugation successfully transferred the gene cassette from the B. subtilis chromosome into the G. kaustophilus allowing for the desired genetic transformation. CONCLUSIONS: This transformation approach described here will provide a new tool to facilitate the flexible genetic manipulation of G. kaustophilus.
Author(s): Mori K, Fukui K, Amatsu R, Ishikawa S, Verrone V, Wipat A, Meijer WJJ, Yoshida K-I
Publication type: Article
Publication status: Published
Journal: Microbial Cell Factories
Online publication date: 08/03/2022
Acceptance date: 19/02/2022
Date deposited: 21/03/2022
ISSN (electronic): 1475-2859
Publisher: BioMed Central Ltd.
PubMed id: 35260160
Altmetrics provided by Altmetric