ePrints

An oomycete NLP cytolysin forms transient small pores in lipid membranes

Lookup NU author(s): Professor Jeremy LakeyORCiD

Downloads

• Published version [.pdf]

Licence

This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

Copyright © 2022 The Authors. Microbial plant pathogens secrete a range of effector proteins that damage host plants and consequently constrain global food production. Necrosis and ethylene-inducing peptide 1–like proteins (NLPs) are produced by numerous phytopathogenic microbes that cause important crop diseases. Many NLPs are cytolytic, causing cell death and tissue necrosis by disrupting the plant plasma membrane. Here, we reveal the unique molecular mechanism underlying the membrane damage induced by the cytotoxic model NLP. This membrane disruption is a multistep process that includes electrostatic-driven, plant-specific lipid recognition, shallow membrane binding, protein aggregation, and transient pore formation. The NLP-induced damage is not caused by membrane reorganization or large-scale defects but by small membrane ruptures. This distinct mechanism of lipid membrane disruption is highly adapted to effectively damage plant cells.

Publication metadata

Author(s): Pirc K, Clifton LA, Yilmaz N, Saltalamacchia A, Mally M, Snoj T, Znidarsic N, Srnko M, Borisek J, Parkkila P, Albert I, Podobnik M, Numata K, Nurnberger T, Viitala T, Derganc J, Magistrato A, Lakey JH, Anderluh G

Publication type: Article

Publication status: Published

Journal: Science Advances

Year: 2022

Volume: 8

Issue: 10

Online publication date: 11/03/2022

Acceptance date: 21/01/2022

Date deposited: 29/03/2022

ISSN (electronic): 2375-2548

Publisher: American Association for the Advancement of Science (AAAS)

URL: https://doi.org/10.1126/sciadv.abj9406

DOI: 10.1126/sciadv.abj9406

PubMed id: 35275729

Altmetrics

Funding

Share