Browse by author
Lookup NU author(s): Charlotte Graham, Dr Rhoda StefanatosORCiD, Dr Filippo Scialo
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022, The Author(s). Mitochondrial reactive oxygen species (mtROS) are cellular messengers essential for cellular homeostasis. In response to stress, reverse electron transport (RET) through respiratory complex I generates high levels of mtROS. Suppression of ROS production via RET (ROS-RET) reduces survival under stress, while activation of ROS-RET extends lifespan in basal conditions. Here, we demonstrate that ROS-RET signalling requires increased electron entry and uninterrupted electron flow through the electron transport chain (ETC). We find that in old fruit flies, ROS-RET is abolished when electron flux is decreased and that their mitochondria produce consistently high levels of mtROS. Finally, we demonstrate that in young flies, limiting electron exit, but not entry, from the ETC phenocopies mtROS generation observed in old individuals. Our results elucidate the mechanism by which ROS signalling is lost during ageing.
Author(s): Graham C, Stefanatos R, Yek AEH, Spriggs RV, Loh SHY, Uribe AH, Zhang T, Martins LM, Maddocks ODK, Scialo F, Sanz A
Publication type: Article
Publication status: Published
Journal: GeroScience
Year: 2022
Volume: 44
Pages: 1961-1974
Print publication date: 01/08/2022
Online publication date: 30/03/2022
Acceptance date: 22/03/2022
Date deposited: 21/04/2022
ISSN (print): 2509-2715
ISSN (electronic): 2509-2723
Publisher: Springer Nature
URL: https://doi.org/10.1007/s11357-022-00555-x
DOI: 10.1007/s11357-022-00555-x
PubMed id: 35355221
Altmetrics provided by Altmetric