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Gut Commensal Bacteroidetes Encode a Novel Class of Vitamin B12-Binding Proteins

Lookup NU author(s): Dr Javier Abellon-RuizORCiD, Professor Bert van den Berg



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2022 Putnam et al. Human gut commensal Bacteroidetes rely on multiple transport systems to acquire vitamin B12 and related cobamides for fitness in the gut. In addition to a set of conserved transport proteins, these systems also include a diverse repertoire of additional proteins with unknown function. Here, we report the function and structural characterization of one of these proteins, BtuH, which binds vitamin B12 directly via a C-terminal globular domain that has no known structural homologs. This protein is required for efficient B12 transport and competitive fitness in the gut, demonstrating that members of the heterogeneous suite of accessory proteins encoded in Bacteroides cobamide transport system loci can play key roles in vitamin acquisition.

Publication metadata

Author(s): Putnam EE, Abellon-Ruiz J, Killinger BJ, Rosnow JJ, Wexler AG, Folta-Stogniew E, Wright AT, van den Berg B, Goodman AL

Publication type: Article

Publication status: Published

Journal: mBio

Year: 2022

Volume: 13

Issue: 2

Print publication date: 01/04/2022

Online publication date: 01/03/2022

Acceptance date: 10/01/2022

Date deposited: 18/05/2022

ISSN (print): 2161-2129

ISSN (electronic): 2150-7511

Publisher: American Society for Microbiology


DOI: 10.1128/mbio.02845-21

PubMed id: 35227073


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Funder referenceFunder name
214222/Z/18/ZWellcome Trust