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Lookup NU author(s): Dr Javier Abellon-RuizORCiD, Professor Bert van den Berg
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022 Putnam et al. Human gut commensal Bacteroidetes rely on multiple transport systems to acquire vitamin B12 and related cobamides for fitness in the gut. In addition to a set of conserved transport proteins, these systems also include a diverse repertoire of additional proteins with unknown function. Here, we report the function and structural characterization of one of these proteins, BtuH, which binds vitamin B12 directly via a C-terminal globular domain that has no known structural homologs. This protein is required for efficient B12 transport and competitive fitness in the gut, demonstrating that members of the heterogeneous suite of accessory proteins encoded in Bacteroides cobamide transport system loci can play key roles in vitamin acquisition.
Author(s): Putnam EE, Abellon-Ruiz J, Killinger BJ, Rosnow JJ, Wexler AG, Folta-Stogniew E, Wright AT, van den Berg B, Goodman AL
Publication type: Article
Publication status: Published
Journal: mBio
Year: 2022
Volume: 13
Issue: 2
Print publication date: 01/04/2022
Online publication date: 01/03/2022
Acceptance date: 10/01/2022
Date deposited: 18/05/2022
ISSN (print): 2161-2129
ISSN (electronic): 2150-7511
Publisher: American Society for Microbiology
URL: https://doi.org/10.1128/mbio.02845-21
DOI: 10.1128/mbio.02845-21
PubMed id: 35227073
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