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Blastococcus tunisiensis sp. nov., isolated from limestone collected in Tunisia

Lookup NU author(s): Dr Carmen Montero-Calasanz, Emeritus Professor Hans-Peter Klenk, Dr Imen Nouioui



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© 2022 The Authors. A new actinobacterium strain, designated BMG 823T, was isolated from a limestone sample collected in Tunisia. Its taxonomic position was scrutinized using a polyphasic approach. Colonies of strain BMG 823T were pink orange-coloured, regular and had a moist surface. Cells are Gram-stain-positive, catalase-negative and oxidase-negative. The strain grew at pH 5.5–9, 10–40 °C and in presence of up to 4% NaCl (w/v). Chemotaxonomically, strain BMG 823T was characterized by cell-wall type III containing meso-diaminopimelic acid as diamino acid, glucose, ribose and rhamnose as whole-cell sugars, MK-9(H4 ) as predominant menaquinone, and phosphatidylcholine, diphosphadidylglycerol, phosphatidethanolamine, phosphatidylcholine, phosphatidylinositol, unidentified glycolipid, unidentified aminophospholipids and unidentified glycophospholipid as major polar lipids. The fatty acid profile consisted of iso-C16:0 and iso-C17:1 ω9. Phylogenetic trees based on 16S rRNA gene and genome sequences placed strain BMG 823T within the genus Blastococcus and separated it from all type strains of validly published species. Comparison of 16S rRNA gene sequence similarity, digital DNA–DNA hybridization and average nucleotide identity indicated that strain BMG 823T was most closely related to Blastococcus litoris DSM 106127T and Blastococcus colisei BMG 822T with pairwise values well below the species differentiation thresholds. The distinct phenotypic and genotypic features of strain BMG 823T (=DSM 46838T=CECT 8881T) within the genus Blastococcus warrant its recognition as the type strain for the new species for which we propose the name Blastococcus tunisiensis sp. nov.

Publication metadata

Author(s): Louati M, Hezbri K, Montero-Calasanz MC, Rohde M, Goker M, Ghodhbane-Gtari F, Klenk H-P, Nouioui I, Gtari M

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2022

Volume: 72

Issue: 6

Online publication date: 23/06/2022

Acceptance date: 07/05/2022

Date deposited: 29/07/2022

ISSN (print): 1466-5026

ISSN (electronic): 1466-5034

Publisher: Microbiology Society


DOI: 10.1099/ijsem.0.005441

ePrints DOI: 10.57711/76d4-ap30


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