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A bioprintable gellan gum/lignin hydrogel: a smart and sustainable route for cartilage regeneration

Lookup NU author(s): Annachiara Scalzone, Dr Piergiorgio GentileORCiD


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© 2022In this work a hydrogel, based on a blend of two gellan gums with different acyl content embedding lignin (up to 0.4%w/v) and crosslinked with magnesium ions, was developed for cartilage regeneration. The physico-chemical characterizations established that no chemical interaction between lignin and polysaccharides was detected. Lignin achieved up to 80 % of ascorbic acid's radical scavenging activity in vitro on DPPH and ABTS radicals. Viability of hMSC onto hydrogel containing lignin resulted comparable to the lignin-free one (>70 % viable cells, p > 0.05). The presence of lignin improved the hMSC 3D-constructs chondrogenesis, bringing to a significant (p < 0.05) up-regulation of the collagen type II, aggrecan and SOX 9 chondrogenic genes, and conferred bacteriostatic properties to the hydrogel, reducing the proliferation of S. aureus and S. epidermidis. Finally, cellularized 3D-constructs were manufactured via 3D-bioprinting confirming the processability of the formulation as a bioink and its unique biological features for creating a physiological milieu for cell growth.

Publication metadata

Author(s): Bonifacio MA, Cometa S, Cochis A, Scalzone A, Gentile P, Scalia AC, Rimondini L, Mastrorilli P, De Giglio E

Publication type: Article

Publication status: Published

Journal: International Journal of Biological Macromolecules

Year: 2022

Volume: 216

Pages: 336-346

Print publication date: 01/09/2022

Acceptance date: 01/07/2022

ISSN (print): 0141-8130

ISSN (electronic): 1879-0003

Publisher: Elsevier B.V.


DOI: 10.1016/j.ijbiomac.2022.07.002

PubMed id: 35798077


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