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Lookup NU author(s): Annachiara Scalzone,
Dr Piergiorgio GentileORCiD
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© 2022In this work a hydrogel, based on a blend of two gellan gums with different acyl content embedding lignin (up to 0.4%w/v) and crosslinked with magnesium ions, was developed for cartilage regeneration. The physico-chemical characterizations established that no chemical interaction between lignin and polysaccharides was detected. Lignin achieved up to 80 % of ascorbic acid's radical scavenging activity in vitro on DPPH and ABTS radicals. Viability of hMSC onto hydrogel containing lignin resulted comparable to the lignin-free one (>70 % viable cells, p > 0.05). The presence of lignin improved the hMSC 3D-constructs chondrogenesis, bringing to a significant (p < 0.05) up-regulation of the collagen type II, aggrecan and SOX 9 chondrogenic genes, and conferred bacteriostatic properties to the hydrogel, reducing the proliferation of S. aureus and S. epidermidis. Finally, cellularized 3D-constructs were manufactured via 3D-bioprinting confirming the processability of the formulation as a bioink and its unique biological features for creating a physiological milieu for cell growth.
Author(s): Bonifacio MA, Cometa S, Cochis A, Scalzone A, Gentile P, Scalia AC, Rimondini L, Mastrorilli P, De Giglio E
Publication type: Article
Publication status: Published
Journal: International Journal of Biological Macromolecules
Print publication date: 01/09/2022
Acceptance date: 01/07/2022
ISSN (print): 0141-8130
ISSN (electronic): 1879-0003
Publisher: Elsevier B.V.
PubMed id: 35798077
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