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Author Correction: MiDAS 4: A global catalogue of full-length 16S rRNA gene sequences and taxonomy for studies of bacterial communities in wastewater treatment plants (Nature Communications, (2022), 13, 1, (1908), 10.1038/s41467-022-29438-7)

Lookup NU author(s): Professor Thomas CurtisORCiD, Professor Dominic Frigon, Dr David Garcia-Casas, Professor David Jenkins



This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).


© The Author(s) 2022.The original version of this Article included the following errors in reference citations: It incorrectly cited ‘Thompson, L. R. et al. A communal catalogue reveals Earth’s multiscale microbial diversity. Nature 551, 457–463 (2017)’ and ‘Peterson, J. et al. The NIH Human Microbiome Project. Genome Res. 19, 2317–2323 (2009)’ as Refs 18 and 19 in the Introduction, at ‘In the MiDAS project, we have thoroughly evaluated different wet-lab protocols, e.g., DNA extraction methods, and choice of amplicon primers and amplicon library preparation17-19.’ The two references have been removed from the correct version. It incorrectly cited ‘Zheng, M. et al. Active ammonia-oxidizing bacteria and archaea in wastewater treatment systems. J. Environ. Sci. 102, 273–282 (2021)’ and ‘Thompson, L. R. et al. A communal catalogue reveals Earth’s multiscale microbial diversity. Nature 551, 457–463 (2017)’ as Refs 42 and 18 in the last paragraph of Results and Discussion, at ‘In the Danish WWTPs, we have successfully done this for groups in the Acidobacteriota42 based on the MiDAS 3 database18.’ The correct version replaces the first reference with ‘Kristensen, J. M., Singleton, C., Clegg, L.-A., Petriglieri, F. and Nielsen, P. H. High diversity and functional potential of undescribed “Acidobacteriota” in Danish wastewater treatment plants. Front. Microbiol. 12, 906 (2021)’ and the second reference with Ref. 16. It incorrectly cited ‘Rosselló-Mora, R. A., Wagner, M., Amann, R. and Schleifer, K. H. The abundance of Zoogloea ramigera in sewage treatment plants. Appl. Environ. Microbiol. 61, 702–707 (1995)’ as Ref. 43 in the last paragraph of Results and Discussion, at ‘Our recent retrieval of more than 1000 high-quality MAGs from Danish WWTPs with advanced process design is also an important step to link identity to function43.’ The correct version replaces this reference with ‘Singleton, C. M. et al. Connecting structure to function with the recovery of over 1000 high-quality metagenome-assembled genomes from activated sludge using long-read sequencing. Nature Communications 12, 2009 (2021)’. It incorrectly cited ‘Nierychlo, M. et al. Candidatus Amarolinea and Candidatus Microthrix are mainly responsible for filamentous bulking in Danish municipal wastewater treatment plants. Front. Microbiol. 11, 1214 (2020)’, ‘Klindworth, A. et al. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 41, 1–11 (2013)’ and ‘Lane, D. J. 16S/23S rRNA sequencing In Nucleic Acid Techniques in Bacterial Systematics (eds Stackebrandt, E. and Goodfellow, M.) (Wiley, 1991)’ as Refs 44–46 in the last paragraph of Results and Discussion, at ‘These MAGs may also form the basis for further studies to link identity and function, e.g., by applying metatranscriptomics44 and other in situ techniques such as FISH combined with Raman45,46.’ The correct version replaces these references with ‘Herold, M. et al. Integration of time-series meta-omics data reveals how microbial ecosystems respond to disturbance. Nature Communications 11, 5281 (2020)’, ‘Fernando, E. Y. et al. Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH. ISME J. 13, 1933–1946 (2019)’ and ‘Petriglieri, F. et al. Quantification of Biologically and Chemically Bound Phosphorus in Activated Sludge from Full-Scale Plants with Biological P-Removal. Environ. Sci. Technol. 56, 5132–5140 (2022)’. The errors have been corrected in the PDF and HTML versions of the Article.

Publication metadata

Author(s): Dueholm MKD, Nierychlo M, Andersen KS, Rudkjobing V, Knutsson S, Arriaga S, Bakke R, Boon N, Bux F, Christensson M, Chua ASM, Curtis TP, Cytryn E, Erijman L, Etchebehere C, Fatta-Kassinos D, Frigon D, Garcia-Chaves MC, Gu AZ, Horn H, Jenkins D, Kreuzinger N, Kumari S, Lanham A, Law Y, Leiknes TO, Morgenroth E, Muszynski A, Petrovski S, Pijuan M, Pillai SB, Reis MAM, Rong Q, Rossetti S, Seviour R, Tooker N, Vainio P, van Loosdrecht M, Vikraman R, Wanner J, Weissbrodt D, Wen X, Zhang T, Nielsen PH, Albertsen M, Nielsen PH

Publication type: Note

Publication status: Published

Journal: Nature Communications

Year: 2022

Volume: 13

Issue: 1

Online publication date: 11/07/2022

Acceptance date: 02/04/2018

ISSN (electronic): 2041-1723

Publisher: Nature Research


DOI: 10.1038/s41467-022-31423-z

PubMed id: 35821016