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Lookup NU author(s): Dr Leonardo Rios Solis
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2022 The Authors. Published by American Chemical Society.As redesigning organisms using engineering principles is one of the purposes of synthetic biology (SynBio), the standardization of experimental methods and DNA parts is becoming increasingly a necessity. The synthetic biology community focusing on the engineering of Saccharomyces cerevisiae has been in the foreground in this area, conceiving several well-characterized SynBio toolkits widely adopted by the community. In this review, the molecular methods and toolkits developed for S. cerevisiae are discussed in terms of their contributions to the required standardization efforts. In addition, the toolkits designed for emerging nonconventional yeast species including Yarrowia lipolytica, Komagataella phaffii, and Kluyveromyces marxianus are also reviewed. Without a doubt, the characterized DNA parts combined with the standardized assembly strategies highlighted in these toolkits have greatly contributed to the rapid development of many metabolic engineering and diagnostics applications among others. Despite the growing capacity in deploying synthetic biology for common yeast genome engineering works, the yeast community has a long journey to go to exploit it in more sophisticated and delicate applications like bioautomation.
Author(s): Malcl K, Watts E, Roberts TM, Auxillos JY, Nowrouzi B, Boll HO, Nascimento CZSD, Andreou A, Vegh P, Donovan S, Fragkoudis R, Panke S, Wallace E, Elfick A, Rios-Solis L
Publication type: Review
Publication status: Published
Journal: ACS Synthetic Biology
Year: 2022
Volume: 11
Issue: 8
Pages: 2527-2547
Print publication date: 19/08/2022
Online publication date: 08/08/2022
Acceptance date: 08/08/2022
ISSN (electronic): 2161-5063
Publisher: American Chemical Society
URL: https://doi.org/10.1021/acssynbio.1c00442
DOI: 10.1021/acssynbio.1c00442
PubMed id: 35939789
