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© 2022 Wiley-VCH GmbH.Vitamin B12 (VB12) plays vital roles as a cofactor in reactions related to biosynthesis and metabolic regulation. Animals with diarrhoea from intestinal inflammation are susceptible to VB12 deficiency due to dysfunctional absorption. No current medications for canine intestinal inflammation can simultaneously act as VB12 supplements. Here we have tested a strain of VB12-producing Lactobacillus, to investigate its safety in healthy dogs and test for hypothesized therapeutic and preventive effects on murine colitis. Results from enzyme-linked immunosorbent assay, histopathological analysis, and quantitative polymerase chain reaction showed normal physical conditions of healthy dogs given Lactobacillus, and blood biochemical indices showed no significant differences in markers, indicating safety of Lactobacillus to healthy dogs. The microbiota in animals receiving VB12-producing Lactobacillus probiotic exhibited decreased abundance of Escherichia coli and concomitant increase in Lactobacillus. The probiotic supplement also resulted in downregulation of proinflammatory cytokines in murine colon tissues, reduced myeloperoxidase activity and malondialdehyde level, and significantly increased serum VB12 level and decreased homocysteine in therapeutic and preventive experiments. Moreover, Lactobacillus supplement decreased colonic inflammation and injury, improved gut microbiota, and ameliorated VB12 deficiency as an adjunctive therapy. We conclude this product is potentially beneficial for efficient therapy and prevention of VB12 deficiency form intestinal inflammation in canine clinical practice.
Author(s): Zhang P, Li B, Mu J, Liu D, Zhang G, Mao X, Huang K, Waldron KJ, Chen X
Publication type: Article
Publication status: Published
Journal: Journal of Animal Physiology and Animal Nutrition
Year: 2022
Volume: 106
Issue: 6
Pages: 1368-1382
Print publication date: 01/11/2022
Online publication date: 31/08/2022
Acceptance date: 05/08/2022
ISSN (print): 0931-2439
ISSN (electronic): 1439-0396
Publisher: John Wiley and Sons Inc
URL: https://doi.org/10.1111/jpn.13767
DOI: 10.1111/jpn.13767
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